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Transformation and Advanced Techniques
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Glow-in-the-Dark Transformation Kit
Click image for a larger view.
Cockroach (Periplaneta), Living, Adults, Pk 12
Rating:
Glow-in-the-Dark Transformation Kit
Note:
This kit is available with perishable materials included (all
items) or with a prepaid coupon you can use to request delivery of perishables at your convenience. Redeem your coupon 24/7 at
www.carolina.com/livingform
, by phone at 800.334.5551, or by fax at 800.222.7112.
Teacher Demonstration Kit and Student Kits include:
E. coli
Plasmid DNA Solution
Transformation Buffer and Luria Broth
Ready-to-Pour Plates (Demonstration Kit contains prepoured plates
)
Ampicillin
(not in Demonstration Kit)
Spreading Beads and Sterile Tubes
Pipets and Transfer Loops
Teacher's Manual with Student Master Sheets
Teacher Demonstration Kit Perishables Refill includes:
E. coli
, Plasmid DNA Solution, Prepoured Plates
.
4- and 8- Station Perishables Refills include:
Ampicillin
,
E. coli
, Plasmid DNA Solution
.
DNALC Glow-in-the-Dark Transformation Kit
Suitable for AP® Biology
No Special equipment is needed to view the bioluminescence
Requires no liquid culturing
Minimal teacher preparation
Note:
Bacteria transformed with the pVIB plasmid must be grown at 30°C or less for the glowing to be seen. Each kit contains a coupon for prepaid delivery of perishable materials. Contact us or return the coupon to request delivery of perishable material. If you need perishables to arrive with the kit, please specify when ordering. Sold only to schools and businesses; cannot be shipped via USPS.
Biotech Kit Instruction Manuals
Whether you're considering this product for your classroom or just need another copy of the manual, you'll find many of our most popular Biotechnology Kit manuals here. The link will open a new browser window, so please disable popup blockers.
Intermediate
- Easy to perform; requires some background knowledge.
Bacteria that produce light are very common in the ocean. One luminescent bacterium is Vibrio fischeri. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.
Note:
Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.
Item #
Product
Qty
Price
211086
Glow-in-the-Dark Transformation Teacher Demo Kit (with Prepaid Coupon)
$51.00
211086P
Glow-in-the-Dark Transformation Teacher Demo Kit (with Perishables)
$51.00
211086C
Glow-in-the-Dark Colony Transformation Teacher Demonstration Kit Perishables Refill
$19.95
211087
Glow-in-the-Dark Transformation 4-Station Student Kit (with Prepaid Coupon)
$64.95
211087P
Glow-in-the-Dark Transformation 4-Station Student Kit (with Perishables)
$64.95
211088
Glow-in-the-Dark Transformation 8-Station Student Kit (with Prepaid Coupon)
$107.00
211088P
Glow-in-the-Dark Transformation 8-Station Student Kit (with Perishables)
$107.00
211088C
Glow-in-the-Dark Transformation 4- and 8-Station Kit Perishables Refill
$21.50
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