Use this kit to teach: Population Genetics Hardy-Weinberg equilibrium Forensics techniques The repeated structure of the VNTR polymorphism makes it more difficult to amplify - so this experiment greatly benefits from a "hot start", in which one of the reagents is added during the first denaturing cycle. Each Kit Contains instructions and materials for 25 students and is available in 2 versions. The first version, for DNA extraction and amplification only, includes Chelex, proteinase K, Ready-to-Go Beads (in 0.2- or 0.5-mL reaction tubes), mineral oil, primer/loading dye mix, and DNA markers. The second version includes all the above plus agarose, electrophoresis buffer, staining trays, gloves, and either ethidium bromide or CarolinaBLU™ stain. Note: Kits contain perishable materials and are shipped on gel cold packs. Sold only to schools and businesses. Needed But Not Included:1.5-mL Microcentrifuge Tubes, 15-mL Conical Tubes, Saline Solution, Paper Cups, Thermal Cycler, Electrophoresis Equipment, Micropipets.

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Introduce students to Hardy-Weinberg equilibrium and the concept of allele frequencies in populations.

• Provides an introduction to the use of PCR in forensics
• Helps demonstrate real-world applications of PCR

This lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Differences in the number of repeated units produce longer and shorter alleles, which can be resolved by gel electrophoresis. Because the VNTR locus has more than 29 different alleles, a panel of student types shows a variety of different genotypes.