Note: This kit is available with perishable materials included (all items) or with a prepaid coupon you can use to request delivery of perishables at your convenience. Redeem your coupon 24/7 at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112. Kit includes: Acinetobacter calcoaceticus MicroKwik Culture® (streptomycin resistant) Acinetobacter calcoaceticus MicroKwik Culture® (streptomycin sensitive) 5 Bottles Heart Infusion Agar Detergent Solution Streptomycin Sulfate Solution Sterile Petri Dishes Dropping Pipets Inoculating Loops Test Tubes with Caps Permanent Marker Autoclave Disposal Bag Teacher Instructions Needed but not included: 37° C Incubator

For a class of 30. Transformation is a form of recombination in which free DNA is released from donor cells, taken up by a recipient’s cells, and incorporated into the recipient’s genome. Students treat streptomycin-resistant bacterial cells with a detergent to lyse the cells, thus releasing the DNA. Then they incubate streptomycin-sensitive bacterial cells in contact with free DNA. The streptomycin-sensitive cells are transformed and can then grow in the presence of streptomycin as streptomycin-resistant cells.

Before rehydration, each MicroKwik Culture® can be kept at room temperature for 2 months or under refrigeration for 6 months.