During the course of the experimental procedure described, up to 8 additional sets of 6 plates each can be easily prepared for distribution to other students. Kit provides enough phage and bacteria for such duplication results, but the required agar plates and soft agar must be purchased separately. Please refer to Bacteriophage Media, item#124730 Plaque Assay Plating set. (Component lists for item #124550} Coliphage Stock Suspensions: 3mL 1 x 108 T4rllA Known Mutant Tester 5mL 1 x 109 T4rll Unknown Mutant No. 1 5mL 1 x 109 T4rll Unknown Mutant No. 2 5mL 1 x 109 T4rll Unknown Mutant No. 3 3mL 1 x 109 T4rllAB Reference Deletion Mutant X 3mL 1 x 109 T4rllAB Reference Deletion Mutant Y 3mL 1 x 109 T4rllAB Reference Deletion Mutant Z Media and Glassware (Sterile): Bottle Nutrient Peptone Broth (80mL) 2 Bottles Base Layer Agar 3 Tubes Broth (5mL) 4 Tubes Broth for 10-Fold Dilutions (9mL) 13 Tubes Broth for 100-Fold Dilutions (9.9mL) 6 Screw-Cap Vials (labeled for genetic crosses) 10 Tubes Soft Agar Dropper Vial (5mL) 13 Transfer Pipets (0.1mL) 22 Transfer Pipets (1mL) 3 Pasteur Pipets (9”) 21 Glass Spotting Rods (150 mm) Sterile Petri Dishes Bacteria Stock Cultures: Agar Slant E. coli B Agar Slant E. coli K12 (lambda) Other Supplies: Wax Marker 3 Bulbs for Pasteur Pipets 10 Flowsheets 10 Manuals with Worksheets

All materials and instructions for (1) conducting complementation (cis-trans) spot tests to place unknown T4rll mutations in either cistron A or B, and for (2) conducting recombination spot tests to determine by deletion mapping the A-segments within which the rllA mutations occur. Exercises are recommended for an individual, a team of 2 or 3 students, or use as a demonstration.

Other students, however, can participate when they are given sets of result plates for personal analysis and conclusions. Results are obtained from 4 spot tests and 2 control plates.

Note: Kit contains coupon for prepaid delivery of perishable materials. Return coupon at least 2 weeks prior to requested elivery date to ensure prompt arrival of materials.