Each kit contains instructions and materials for 25 students, and is available in 2 versions. The First Version, for DNA extraction and amplification only, includes Chelex, proteinase K, Ready-to-go-Beads, (in 0.2- or 0.5-mL reaction tubes), primer/loading dye mix, mineral oil, and DNA markers. The Second Version includes all of the above, plus agarose, electrophoresis buffer, staining trays, gloves, and either ethigium bromide or CarolinaBLU stain. Needed But Not Included: 3 Water baths or Thermal Cycler, Electrophoresis Equipment, Micropipettors, 1.5-mL Mictocentrifuge Tubes, 15-mL Conical Tubes, Saline Solution, Paper Cups. Note: Kits contain perishable materials and are shipped on gel cold packs. Sold only to schools and businesses.

Polymerase Chain Reaction - Human DNA PCR Kits Each kit uses a 30-minute method that requires no specialized equipment to isolate template DNA from hair sheaths or cheek (buccal) cells Simply add student DNA template and primer mix to the ready-to-go Beads™ which incorporate the needed components and are stable at room temperature. Saves time and ensures accuracy To save additional time, loading dye is incorporated into the primer mix - amplified reactions can be taken from the thermal cycler and immediately loaded for gel electrophoresis. (Human haplotyping kit requires that loading dye be added after the final step). PCR products can be readily analyzed on agarose and can be stained with either ethidium bromide or CarolinaBLU™ Why Carolina™ PCR Kits? Highest Quality - Carolina's staff works with the experts at the Dolan DNA Learning Center (DNALC) of Cold Spring Harbor and other collaborators to develop the very best kits available. Teacher and Student Friendly - We've taken measures to save you prep time and make the labs convenient Carolina™ Service and Guarantee - The best assurance you can have. We offer free online and telephone support. If you have a question, we'll answer it promptly. If you have a problem with any of our materials, we'll do whatever it takes to fix it - guaranteed!

Biotech Kit Instruction Manuals Whether you're considering this product for your classroom or just need another copy of the manual, you'll find many of our most popular Biotechnology Kit manuals here. The link will open a new browser window, so please disable popup blockers.

Advanced - For experienced high school and college classes; requires some technical skill.

• Simple, robust protocol is ideal for the classroom
• Enlivens discussions on the molecular basis of heredity and evolution
• Provides a basic version of the lab that is used in forensics and the analysis of ancient DNA samples
• Teaches real-world applications of PCR
• Includes online resources to enhance the learning experience

Students use safe saline mouthwash or hair and Chelex extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome, which contains numerous single nucleotide polymorphisms (SNPs).

After gel electrophoresis confirms amplification, the student samples may be sent for sequencing by the Dolan DNA Learning Center, and the results are posted on the DNALC's Sequence Server (http://www.bioservers.org/bioserver/index1.html).