Kit includes: DNA Sequencing Chart (24 × 36") and DNA Sequence Pages 6 Mock Sequencing Gels and Weigh Boats pWEB Plasmid DNA EcoRI, PstI, and Restriction Buffer Tube Rack and Loading Dye Agarose and TBE Buffer CarolinaBLU™ Gel and Buffer Stain CarolinaBLU™ Final Stain and Staining Trays Microcentrifuge Tubes and Disposable Gloves Teacher and Student Instructions Needed but not supplied: Transparent Tape and Scissors Computer with Internet Access Water Bath (37° C) and Micropipets Gel Electrophoresis Chambers and Power Supplies

This unique kit lets your students experience how computers are used in a biotechnology research laboratory.

In Module 1, you and your students do 2 dry labs designed to introduce DNA sequencing.

In Module 2, students log onto the Internet to use our Webcutter software, which interfaces with the National Center for Biotechnology Information Web site, to determine both the identity of their gene fragment and its restriction map.

In Module 3, students take the plasmid containing their gene fragment and do 2 restriction enzyme digests and gel electrophoresis to confirm the identity of their unknown gene.

Materials are sufficient for 6 teams of students.

Note: Kit contains coupon for prepaid delivery of perishables, unless you request delivery of perishables with kit. Contact us or return at least 2 weeks prior to requested delivery date to ensure prompt arrival of materials. Foreign orders require special handling.