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Students take a hands-on and visual approach to understanding the activity of the enzyme amylase in a variety of samples. Students can design their own investigation by choosing to isolate their amylase from saliva, bean extract, or a variety of other samples. After running their protein samples on the gel, they observe starch breakdown to determine the location of amylase bands. This advanced activity is the perfect inquiry component for your next protein lab.
AdvancedFor experienced high school and college classes; requires some technical skill.
Good for inquiry-based learning!
To combine protein electrophoresis with an understanding of enzyme activity, students use agarose protein electrophoresis to separate proteins in human saliva, bean extract, or another sample of interest and examine them for amylase activity.
Samples are run in duplicate: Half the gel is used for COOMASSIE® total protein staining to show multiple proteins; the other half is incubated under conditions to optimize amylase activity. Visible gel clearing from digestion of starch present in the gel can be seen where the bands of amylase are present.
Note: Kit is shipped with perishable materials.
|Pure Alpha Amylase, 0.5 g||1||Included|
|Non-Reducing Protein Loading Dye, 350 µL||1||Included|
|COOMASSIE® Protein Stain Solution, 500 mL||1||Included|
|COOMASSIE® 5x Destain Solution, 500 mL||1||Included|
|Triton X-100 Solution, 30 mL||1||Included|
|Bush Beans, 34 g||1||Included|
|5x Enzyme Running Buffer, 350 mL||1||Included|
|Melt ’n Pour Starch Agarose, 350 mL||1||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Electrophoresis Chambers||Needed, Not Included|
|Electrophoresis Power Supplies||Needed, Not Included|
|Micropipets and Tips, 1001,000 µL||Needed, Not Included|
|Micropipets and Tips, 110 µL or 1–20 µL||Needed, Not Included|
|Microcentrifuge Tubes||48||Needed, Not Included|
|70° C Water Bath (optional)||Needed, Not Included|
|Mortar and Pestle (optional)||Needed, Not Included|
|Distilled Water||Needed, Not Included|