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Amylase Electrophoresis Kit (with prepaid coupon]

Item # 211280

Students take a hands-on and visual approach to understanding the activity of the enzyme amylase in a variety of samples. Students can design their own investigation by choosing to isolate their amylase from saliva, bean extract, or a variety of other samples. After running their protein samples on the gel, they observe starch breakdown to determine the location of amylase bands. This advanced activity is the perfect inquiry component for your next protein lab.



In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

Good for inquiry-based learning!
To combine protein electrophoresis with an understanding of enzyme activity, students use agarose protein electrophoresis to separate proteins in human saliva, bean extract, or another sample of interest and examine them for amylase activity.

Samples are run in duplicate: Half the gel is used for COOMASSIE® total protein staining to show multiple proteins; the other half is incubated under conditions to optimize amylase activity. Visible gel clearing from digestion of starch present in the gel can be seen where the bands of amylase are present.

Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishable materials.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • Limited Qty: Flammable Liquid - This product is/contains a limited quantity of a DOT regulated hazardous material. This item will not incur a hazmat fee when shipped by ground. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Pure Alpha Amylase, 0.5 g 1 Included 
Non-Reducing Protein Loading Dye, 350 µL 1 Included
COOMASSIE® Protein Stain Solution, 500 mL 1 Included
COOMASSIE® 5x Destain Solution, 500 mL 1 Included
Staining Trays 25 Included
Latex Gloves 12 Included
Triton X-100 Solution, 30 mL 1 Included
Bush Beans, 34 g 1 Included
5x Enzyme Running Buffer, 350 mL 1 Included
Melt ’n Pour Starch Agarose, 350 mL 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Micropipets and Tips, 100–1,000 µL Needed, Not Included
Micropipets and Tips, 1–10 µL or 1–20 µL Needed, Not Included
Microcentrifuge Tubes 48 Needed, Not Included
70° C Water Bath (optional) Needed, Not Included
Mortar and Pestle (optional) Needed, Not Included
Distilled Water Needed, Not Included
Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551