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Sickle-cell anemia is a classic classroom example of the overarching impact of a single point mutation in the human genetic code. The activity allows your students to further explore this human disease while they learn about protein structure, genetic inheritance, and human evolution. Because the lab requires no additional staining time and uses recombinant hemoglobin samples, it is one of the fastest, safest, and most realistic ways to bring protein electrophoresis into your classroom.
IntermediateEasy to perform; requires some background knowledge.
Need a new way to help students make connections between the impact of mutations on protein structure? Or looking for labs that make real-world disease connections and link to larger concepts such as evolution? Either way, the Sickle-Cell Anemia Protein Electrophoresis Kit is perfect for your biology classroom. Students work with real-world samples, recombinant hemoglobin (poses zero pathogen risk), to conduct a genetic screen on a family and make determinations about the family’s disease state. As a bonus, the kit works on agarose gels and does not require in-class staining, saving you money and time over typical protein electrophoresis kits. This 4-Station Kit is designed for students working in groups of 2 to 4. Note: Kit includes a prepaid coupon to request the perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials.
|Hemoglobin A Control, Tube||4||Included|
|Hemoglobin S Control, Tube||4||Included|
|Hemoglobin Maternal Sample, Tube||4||Included|
|Hemoglobin Paternal Sample, Tube||4||Included|
|Hemoglobin Twin 1 Sample, Tube||4||Included|
|Hemoglobin Twin 2 Sample, Tube||4||Included|
|5x Protein Loading Dye, 600 µL||1||Included|
|Fine Sieving Agarose, 5 g||1||Included|
|5x Tris-Glycine Buffer, 600 mL||1||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Containers of Crushed Ice||4||Needed, Not Included|
|Electrophoresis Chambers with Gel Trays||4||Needed, Not Included|
|Flask, 1 L||1||Needed, Not Included|
|Distilled or Deionized Water, 3.5 L||1||Needed, Not Included|
|Microcentrifuge Tubes||3||Needed, Not Included|
|Flask, 3 L||1||Needed, Not Included|
|Electrophoresis Power Supplies||2||Needed, Not Included|
|Micropipettes and Tips||Needed, Not Included|
|Graduated Cylinder, 2 L||Needed, Not Included|
|Hot Plate, Microwave, or Water Bath||Needed, Not Included|
|White Light Box||Needed, Not Included|
|Heat-Resistant Gloves||Needed, Not Included|
|Aluminum Foil or Parafilm®||Needed, Not Included|
|Microcentrifuge (optional)||Needed, Not Included|
|Camera (optional)||Needed, Not Included|