Login or Register

800.334.5551 Live Chat (offline)

Carolina LabSheets™: Bacterial Hydrolysis of Lipids

Download PDF

In this lab, students culture two species of bacteria on agar medium that contains an emulsion of plant oils and the dye, sprit blue, which forms a complex with the triglycerides of the oils and gives an opaque blue color to the agar. Colonies of bacteria that can secrete lipase, an enzyme that hydrolyzes triglycerides, develop a light area or “halo” in the surrounding medium, due to the diffusion of lipase into the medium and the resultant breakdown of the oil/spirit blue complex.

If the PDF does not display below, you may also download it here.

 

You May Also Like

Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551