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Introduction to Biotechnology: An Essential Curriculum - Vocabulary Test

This vocabulary assessment is used as a pretest to determine your initial knowledge of biotechnology and as a posttest to determine your end-of-course knowledge. Write brief answers that define and explain the following terms. GOOD LUCK!

1.
 
3' end
 
2.
 
37° C
 
3.
 
42° C
 
4.
 
5' end
 
5.
 
95° C - 58° C - 72° C
 
6.
 
adenine
 
7.
 
adhesion zones
 
8.
 
agar
 
9.
 
agarose
 
10.
 
alleles
 
11.
 
amino acid
 
12.
 
ampicillin
 
13.
 
amplification
 
14.
 
antibiotic
 
15.
 
arrayed library
 
16.
 
aseptic technique
 
17.
 
ATP
 
18.
 
autoradiography
 
19.
 
autosome
 
20.
 
bacteriophage
 
21.
 
balance
 
22.
 
BamHI
 
23.
 
bands
 
24.
 
base pair
 
25.
 
base sequence
 
26.
 
base sequence analysis
 
27.
 
bioinformatics
 
28.
 
biotechnology
 
29.
 
bp
 
30.
 
buffer
 
31.
 
CaCl2
 
32.
 
CarolinaBlu™
 
33.
 
casting tray
 
34.
 
cDNA
 
35.
 
centimorgan
 
36.
 
centromere
 
37.
 
chromosome
 
38.
 
clone bank
 
39.
 
clones
 
40.
 
cloning
 
41.
 
cloning vector
 
42.
 
cM
 
43.
 
codon
 
44.
 
competent cells
 
45.
 
complementary base
 
46.
 
conjugation
 
47.
 
conserved sequences
 
48.
 
contig map
 
49.
 
contigs
 
50.
 
control
 
51.
 
cosmid
 
52.
 
crossing over
 
53.
 
culture tube
 
54.
 
cytosine
 
55.
 
denaturation
 
56.
 
diploid
 
57.
 
DNA
 
58.
 
DNA extraction
 
59.
 
DNA replication
 
60.
 
DNA sequence
 
61.
 
DNA spooling
 
62.
 
dNTPs
 
63.
 
domain
 
64.
 
double helix
 
65.
 
E. coli
 
66.
 
EcoRI
 
67.
 
electrophoresis
 
68.
 
electrophoresis chamber
 
69.
 
endonuclease
 
70.
 
enzyme
 
71.
 
ethidium bromide
 
72.
 
eukaryote
 
73.
 
exogenous DNA
 
74.
 
exon
 
75.
 
exonuclease
 
76.
 
experimental factor
 
77.
 
expression
 
78.
 
forensic DNA
 
79.
 
gamete
 
80.
 
gel
 
81.
 
gene
 
82.
 
gene expression
 
83.
 
gene families
 
84.
 
gene library
 
85.
 
gene mapping
 
86.
 
gene mining
 
87.
 
gene product
 
88.
 
gene splicing
 
89.
 
genetic code
 
90.
 
genetics
 
91.
 
genome
 
92.
 
guanine
 
93.
 
haploid
 
94.
 
heterozygosity
 
95.
 
HindIII
 
96.
 
homeobox
 
97.
 
homologies
 
98.
 
homologous chromosomes
 
99.
 
human gene therapy
 
100.
 
Human Genome Project
 
101.
 
hybridization
 
102.
 
hydrogen bonds
 
103.
 
in situ hybridization
 
104.
 
in vitro
 
105.
 
incubation
 
106.
 
inoculating loop
 
107.
 
interface
 
108.
 
interphase
 
109.
 
intron
 
110.
 
jumping gene
 
111.
 
karyotype
 
112.
 
kb
 
113.
 
lambda
 
114.
 
lanes
 
115.
 
LB
 
116.
 
ligase
 
117.
 
linkage
 
118.
 
linkage map
 
119.
 
loading dye
 
120.
 
locus
 
121.
 
marker
 
122.
 
Mb
 
123.
 
meiosis
 
124.
 
metaphase
 
125.
 
microcentrifuge
 
126.
 
microliter
 
127.
 
micropipettor
 
128.
 
mitosis
 
129.
 
MM 294
 
130.
 
mRNA
 
131.
 
mtDNA
 
132.
 
mutation
 
133.
 
nick
 
134.
 
nitrogenous base
 
135.
 
nucleic acid
 
136.
 
nucleotide
 
137.
 
oncogene
 
138.
 
operon
 
139.
 
ori
 
140.
 
palindrome
 
141.
 
pAMP
 
142.
 
Parafilm®
 
143.
 
PCR
 
144.
 
pGREEN
 
145.
 
phage
 
146.
 
photodocumentation
 
147.
 
physical map
 
148.
 
PKU
 
149.
 
plasmid
 
150.
 
polyacrylamide gel
 
151.
 
polyadenation
 
152.
 
polygenetic disorder
 
153.
 
polymerase
 
154.
 
polymorphism
 
155.
 
power supply
 
156.
 
primer
 
157.
 
probe
 
158.
 
prokaryote
 
159.
 
promoter
 
160.
 
protein
 
161.
 
purine
 
162.
 
pyrimidine
 
163.
 
rDNA
 
164.
 
regulatory sequence
 
165.
 
restriction enzyme
 
166.
 
RFLP
 
167.
 
ribosome
 
168.
 
RNA
 
169.
 
rRNA
 
170.
 
sequencing
 
171.
 
sex chromosome
 
172.
 
somatic cells
 
173.
 
Southern blotting
 
174.
 
staining
 
175.
 
staining tray
 
176.
 
STR
 
177.
 
tandem repeat
 
178.
 
taq polymerase
 
179.
 
TBE
 
180.
 
TE
 
181.
 
telomere
 
182.
 
thermocycler
 
183.
 
thymine
 
184.
 
transcription
 
185.
 
transfer pipet
 
186.
 
transformation
 
187.
 
transformation tube
 
188.
 
transillumination system
 
189.
 
translation
 
190.
 
tRNA
 
191.
 
ultraviolet light
 
192.
 
uptake
 
193.
 
uracil
 
194.
 
vector
 
195.
 
virus
 
196.
 
VNTR
 
197.
 
water bath
 
198.
 
wells
 
199.
 
YAC
 
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