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Advanced Bacterial Conjugation Kit (with prepaid coupon)

Item # 211127

Teach your students about gene transfer between bacterial strains. In 1 of the included strains of E. coli, the gene for antibiotic resistance for streptomycin is located on the chromosome. In the other, the gene for antibiotic resistance for ampicillin occurs as extrachromosomal plasmid DNA. Through the process of streaking the conjugated bacteria on 4 different types of antibiotic selective media, students determine which bacterial strain is the donor strain. Materials for 16–32 students.

Price

$115.00

In stock and available to ship.

Intermediate—Easy to perform; requires some background knowledge.

  • Designed for 16 to 32 students (8 stations)
  • Minimal teacher preparation
  • Inquiry-based learning

Teach your students about gene transfer between bacterial strains. In 1 of the included strains of E. coli, the gene for antibiotic resistance for streptomycin is located on the chromosome. In the other, the gene for antibiotic resistance for ampicillin occurs as extrachromosomal plasmid DNA. Through the process of streaking the conjugated bacteria on 4 different types of antibiotic selective media, students determine which bacterial strain is the donor strain. Kit includes prepaid coupon to request perishable materials later at your convenience. Contact us or return coupon to request delivery of perishable materials.

Note: Antibiotic solutions and cultures last 1 month when refrigerated.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli Strain I (Str) 1 Included
E. coli Strain II (Amp and Nal) 1 Included
Naladixic Acid Solution, 0.5%, 4 mL 1 Included
Streptomycin Solution, 2%, 4 mL 3 Included
Ampicillin Solution, 1%, 4 mL 3 Included
Luria Broth, 10 mL 8 Included
Luria Broth Agar, 400 mL 5 Included
Sterile Culture Plates 85 Included
Sterile Loops 100 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual and Reproducible Student Guide 1 Included
Laboratory Markers 8 Needed, Not Included
Incubator, 37° C (optional) 1 Needed, Not Included
Boiling Water Bath (or microwave) 1 Needed, Not Included
Masking Tape Needed, Not Included
Biohazard Disposable Bags Needed, Not Included
Solution of Household Bleach, 10% Needed, Not Included
Parafilm® or Plastic Wrap Needed, Not Included
Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551