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AP Biology Lab 6: Molecular Biology - Module 1 (transformation) Teacher Demonstration Kit (with Prepaid Coupon)

Item # 211080 Exclusive



In stock and available to ship.

This kit is great for teaching:

  • The molecular basis of heredity
  • The relationship between genes and proteins
  • How E. coli is used to engineer proteins useful in medicine and manufacturing
The lab is set up in 3 modules that can be purchased separately and used in conjunction with one another.
Module 1, the Green Gene Colony Transformation Kit, is necessary for creating the GFP-expressing bacteria used in the protein purification exercise in Module 2.
Module 2 contains all the reagents and instructions necessary for purifying GFP from E. coli.
Module 3 contains the acrylamide gels and other electrophoresis reagents necessary for electrophoretic analysis of the purified GFP. The 3rd module is optional but increases the teaching value of the lab.

** Kit and perishable components will ship separately and may arrive on different days.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses.
  • For a limited time, buy 4 Painted Lady Butterfly 5-Larvae Cultures for the price of 3! Click here for details.

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli Culture 1 Included
pGREEN Plasmid Solution, 200 µL 1 Included
LB Agar Plates, Pre-poured 3 Included
LB Agar Plates with Ampicillin, Pre-poured 2 Included
50 mM Calcium Chloride, 3 mL 1 Included
LB Broth, 3 mL 1 Included
Transformation Tubes, 15 mL 2 Included
Sterile Transfer Pipets, 1 mL 7 Included
Glass Beads, 1 oz 1 Included
Sterile Transfer Loops 4 Included
Wire Inoculating Loop 1 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Beaker, 600 mL 1 Needed, Not included
Container (for glass beads) 1 Needed, Not included
Culture Tube Rack 1 Needed, Not included
Wax Marker 1 Needed, Not included
Bunsen Burner 1 Needed, Not included
Water Bath, 42°C 1 Needed, Not included
Autoclavable Disposable Bag 1 Needed, Not included
Ice Needed, Not included
Bleach Solution, 10% Needed, Not included
Incubator (optional) Needed, Not included
AP Biology Lab 6: Molecular Biology - Module 1 (transformation) Teacher Demonstration Kit (with Prepaid Coupon) 5 5 4 5
Great Buy This product produces great results! All of the plates worked without any hitches. Only catch- the materials don't explain what a plasmid is and what markers are on them that allow this experiment to occur. Make sure you prep your class on transformations beforehand. April 19, 2010
Great Project I teach a supplemental high school science class for a federal education program. I was attracted to this project because it is very engaging (what could be more cool for students than transforming bacteria to glow in the dark?) and can be used to reinforce many concepts. It is a great project for the investment. November 16, 2009
Clear, Concise, Understandable Doing research, I have run many transformation experiments - used many kits - had many failures. The kit provided by Carolina is easy for students to follow and they always obtain appropriate results. The kit is sensitive enough that they need to complete the steps in order, however it allows for small errors that produce usable results. I have been very pleased with the Carolina kit! November 10, 2009
great green gene kit A good demonstration of transformation, very well designed and put together. More than enough material to pour plates. The only problem I have found is the melting of the agar; I have tried both microwave and hot water bath, and it's always difficult to completely melt the stuff. This step takes well over the time estimated on the instructions. I still end up having some plates with a lump of unmelted agar. February 9, 2009
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