Login or Register

800.334.5551 Live Chat (offline)

E-Z Gene Splicer DNA Recombination and Transformation 6 Station Kit (with Prepaid Coupon)

Item # 211162

Developed in cooperation with the DNA Learning Center, this lab features a deceptively simple experiment that shows the key elements of constructing recombinant DNA. Students ligate (join) DNA fragments containing 2 types of antibiotic-resistant genes and insert the recombined DNA into E. coli cells using rapid colony transformation. Cells containing recombined DNA molecules are detected by their dual antibiotic resistance.

Price

$200.95

In stock and available to ship.

Advanced - For experienced high school and college classes; requires some technical skill.

Developed in cooperation with the DNA Learning Center, this lab features a deceptively simple experiment that shows the key elements of constructing recombinant DNA. Students ligate (join) DNA fragments containing 2 types of antibiotic-resistant genes and insert the recombined DNA into E. coli cells using rapid colony transformation. Cells containing recombined DNA molecules are detected by their dual antibiotic resistance.

Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. An ampicillin-sensitive strain of E. coli, incapable of producing β-galactosidase for lactose breakdown, is induced to take up pBLU® plasmid DNA. This plasmid contains genes for both ampicillin resistance and β-galactosidase production. The transformed cells are plated on (1) medium containing ampicillin, and (2) medium containing ampicillin and X-gal. (X-gal is a histochemical substrate that, when cleaved by β-galactosidase, forms a blue product.) Transformants appear as white colonies on the ampicillin medium and as blue colonies on the ampicillin/X-gal medium. The transformed colonies' phenotype is lac+ and ampr.

Kit includes a prepaid coupon to request perishables later at your convenience. Contact us or return coupon to request delivery of perishables.

Note: Perishables are shipped on cold gel packs. Foreign orders require special handling.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

Product Families Featuring This Item:

 

This kit includes a prepaid coupon you can use to request delivery of perishables at your convenience. Redeem your coupon 24/7 at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Plasmid DNA Fragments Included
E. coli Included
Ligation Solution Included
Kanamycin Plates 2 Included
Ampicillin Plates 2 Included
Transformation Buffer and LB Broth Included
Ready-to-Pour Plates Included
Kanamycin Included
Ampicillin Included
Spreading Beads Included
Sterile Tubes and Pipets Included
Transfer Loops Included
Teacher’s Manual with Student Masters Included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551