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Glow-in-the-Dark Transformation 8-Station Student Kit (with Prepaid Coupon)

Item # 211088

Bacteria that produce light are very common in the ocean. One luminescent bacterium is Vibrio fischeri. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.

Price

$120.00

In stock and available to ship.

Intermediate - Easy to perform; requires some background knowledge.

Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Kit includes a prepaid coupon to request living materials later at your convenience. Contact us or return the coupon to request delivery of living materials. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli Culture 1 Included
pVIB Plasmid Solution, 200 µL 1 Included
Ampicillin, 4 mL 1 Included
Sterile LB Agar, 400 mL 2 Included
Sterile Petri Dishes 40 Included
50 mM Calcium Chloride, 3 mL 8 Included
LB Broth, 3 mL 8 Included
Transformation Tubes, 15 mL 16 Included
Sterile Transfer Pipets, 1 mL 48 Included
Glass Beads, 1 oz 1 Included
Sterile Transfer Loops 28 Included
Wire Inoculating Loop 1 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Boiling Water Bath or Microwave (for melting agar) 1 Needed, Not included
Beakers, 600 mL 8 Needed, Not included
Container (for glass beads) 1 Needed, Not included
Culture Tube Racks 8 Needed, Not included
Wax Markers 8 Needed, Not included
Masking Tape 1 Needed, Not included
Bunsen Burner 1 Needed, Not included
Water Bath, 42°C 1 Needed, Not included
Autoclavable Disposable Bag 1 Needed, Not included
Ice Needed, Not included
Parafilm® or Plastic Wrap Needed, Not included
Bleach Solution, 10% Needed, Not included
Incubator (optional) Needed, Not included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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