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Bacteria that produce light are very common in the ocean. One luminescent bacterium is Vibrio fischeri. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.
Intermediate - Easy to perform; requires some background knowledge.
Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Kit includes a prepaid coupon to request living materials later at your convenience. Contact us or return the coupon to request delivery of living materials. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.
|E. coli Culture||1||Included|
|pVIB Plasmid Solution, 200 µL||1||Included|
|LB Agar Plates, Pre-poured||3||Included|
|LB Agar Plates with Ampicillin, Pre-poured||2||Included|
|50 mM Calcium Chloride, 3 mL||1||Included|
|LB Broth, 3 mL||1||Included|
|Transformation Tubes, 15 mL||2||Included|
|Sterile Transfer Pipets, 1 mL||7||Included|
|Glass Beads, 1 oz||1||Included|
|Sterile Transfer Loops||4||Included|
|Wire Inoculating Loop||1||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Beaker, 600 mL||1||Needed, Not included|
|Container (for glass beads)||1||Needed, Not included|
|Culture Tube Rack||1||Needed, Not included|
|Wax Marker||1||Needed, Not included|
|Bunsen Burner||1||Needed, Not included|
|Water Bath, 42°C||1||Needed, Not included|
|Autoclavable Disposable Bag||1||Needed, Not included|
|Ice||Needed, Not included|
|Bleach Solution, 10%||Needed, Not included|
|Incubator (optional)||Needed, Not included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.