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Introductory Bacterial Conjugation Kit (with perishables)

Item # 211125P

Use to teach bacterial identification and the intercellular transfer of genes for antibiotic resistance between microorganisms. Students identify and confirm the identity of bacteria with different antibiotic resistance by streaking on different antibiotic selective media. Strains are then combined on a "mating" plate to allow conjugation. Results are tested on various media for growth response to demonstrate that DNA can be transferred between 2 genetically different cells. For 16–32 students.

Price

$100.00

In stock and available to ship.

Intermediate—Easy to perform; requires some background knowledge.

Developed in cooperation with Dr. Robert Thomson, formerly of Marquette University. Useful for teaching bacterial identification and the intercellular transfer of genes for antibiotic resistance between microorganisms. Students identify and confirm the identity of bacteria with different antibiotic resistance by streaking on different antibiotic selective media. Strains are then combined on a “mating” plate to allow conjugation. Results are tested on various media for growth response to demonstrate that DNA can be transferred between 2 genetically different cells. Requires an overnight culture and minimal teacher preparation. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).

Note: Kit includes perishable materials. Kit and perishable components will ship separately and may arrive on different days. Antibiotic solutions and cultures last 1 month when refrigerated.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes perishable materials (all   items). Please select a delivery date during Checkout.

Components Qty Included?
E. coli Strain I (Str)  1 Included
E. coli Strain II (Amp and Nal)  1 Included
Streptomycin Solution, 2%, 4 mL  2 Included
Ampicillin Solution, 1%, 4 mL  2 Included
Luria Broth, 10 mL 8 Included
Luria Broth Agar, 400 mL 4 Included
Sterile Culture Plates 70 Included
Sterile Loops 100 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Laboratory Markers 8 Needed, Not Included
Incubator, 37° C (optional) 1 Needed, Not Included
Boiling Water Bath (or microwave) 1 Needed, Not Included
Masking Tape Needed, Not Included
Biohazard Disposable Bags Needed, Not Included
Solution of Household Bleach, 10% Needed, Not Included
Parafilm® or Plastic Wrap Needed, Not Included
Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551