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Introductory Bacterial Conjugation Kit (with prepaid coupon)

Item # 211125

Students identify and confirm the identity of bacteria with different antibiotic resistance by streaking on different antibiotic selective media. Strains are then combined on a “mating” plate to allow conjugation. Results are tested on various media for growth response to demonstrate that DNA can be transferred between 2 genetically different cells. Requires an overnight culture and minimal teacher preparation. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).

Price

$105.00

In stock and available to ship.

Intermediate—Easy to perform; requires some background knowledge.

Developed in cooperation with Dr. Robert Thomson, formerly of Marquette University. Useful for teaching bacterial identification and the intercellular transfer of genes for antibiotic resistance between microorganisms. Students identify and confirm the identity of bacteria with different antibiotic resistance by streaking on different antibiotic selective media. Strains are then combined on a “mating” plate to allow conjugation. Results are tested on various media for growth response to demonstrate that DNA can be transferred between 2 genetically different cells. Requires an overnight culture and minimal teacher preparation. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).

Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Antibiotic solutions and cultures last 1 month when refrigerated.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli Strain I (Str) 1 Included
E. coli Strain II (Amp and Nal) 1 Included
Streptomycin Solution, 2%, 4 mL 2 Included
Ampicillin Solution, 1%, 4 mL 2 Included
Luria Broth, 10 mL 8 Included
Luria Broth Agar, 400 mL 4 Included
Sterile Culture Plates 70 Included
Sterile Loops 100 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Laboratory Markers 8 Needed, Not Included
Incubator, 37° C (optional) 1 Needed, Not Included
Boiling Water Bath (or microwave) 1 Needed, Not Included
Masking Tape Needed, Not Included
Biohazard Disposable Bags Needed, Not Included
Solution of Household Bleach, 10% Needed, Not Included
Parafilm® or Plastic Wrap Needed, Not Included
Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551