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pBLU® Colony Transformation 4-Station Kit (with perishables)

Item # 211145YP

Designed to match traditional AP® Biology Lab 6. Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. The 4-Station Kit includes enough materials for 4 teams of students. Includes perishable materials when kit is shipped.

Price

$92.50

In stock and available to ship.

Thanks to the dramatic color change and ampicillin, students clearly see the results of introducing new genes into live bacteria

  • Great for teaching the molecular basis of inheritance
  • Simple, straightforward procedure
  • Minimizes teacher prep time
Designed to match traditional AP® Biology Lab 6. Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. Transformants appear as white colonies on the ampicillin medium and as dramatically blue colonies on the ampicillin/X-gal medium. The 4-Station Kit includes enough materials for 4 teams of students. Includes perishable materials when kit is shipped.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

Product Families Featuring This Item:

 

This item ships with living or perishable materials (all items). Please select a delivery date during Checkout.

Components Qty Included?
E. coli Culture  1 Included
pBLU® Plasmid Solution, 200 µL  1 Included
Ampicillin, 4 mL  1 Included
Ampicillin/X-gal Solution, 2% in DMF, 5.4 mL  1 Included
Sterile LB Agar, 400 mL 2 Included
Sterile Petri Dishes 25 Included
50 mM Calcium Chloride, 3 mL 4 Included
LB Broth, 3 mL 4 Included
Transformation Tubes, 15 mL 8 Included
Sterile Transfer Pipets, 1 mL 24 Included
Glass Beads, 1 oz 1 Included
Sterile Transfer Loops 15 Included
Wire Inoculating Loop 1 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Boiling Water Bath or Microwave (for melting agar) 1 Needed, Not included
Beakers, 600 mL 4 Needed, Not included
Container (for glass beads) 1 Needed, Not included
Culture Tube Racks 4 Needed, Not included
Wax Markers 4 Needed, Not included
Masking Tape 1 Needed, Not included
Bunsen Burner 1 Needed, Not included
Water Bath, 42°C 1 Needed, Not included
Autoclavable Disposable Bag 1 Needed, Not included
Ice Needed, Not included
Parafilm® or Plastic Wrap Needed, Not included
Bleach Solution, 10% Needed, Not included
Incubator (optional) Needed, Not included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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