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pClone: Exploring Promoters with Synthetic Biology Kit (with perishables)

Item # 211150P Exclusive

Give your students the opportunity to learn and explore transcription regulation right in your classroom. This unique approach to synthetic biology was developed by college professors focused on creating a unique activity to demonstrate gene regulation. This multi-part lab will expose students to cloning, restriction enzymes, transformation, microbiology, and so much more in an effective classroom protocol.

Price

$215.00

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

  • Unique introduction to synthetic biology
  • Hands-on exploration of the role of promoters in gene regulation
  • Seamlessly blends together multiple areas of biology
  • Takes a traditional transformation lab to the next level
  • Lesson can be facilitated in 6 lab sessions, at most

Bring the unique area of synthetic biology into your classroom with this one-of-a-kind kit activity. Your students get the opportunity to take on the role of genetic engineers as they clone promoters into 2 different plasmids. This hands-on approach to transcription regulation exposes your class to a wide array of biological disciplines including biotechnology, molecular biology, and evolutionary biology.

Students clone the promoters into the plasmids, pClone Red and pClone Blue, using a common cloning technique, Golden Gate Assembly (GGA). After cloning in the promoters, students are tasked with performing a transformation on E. coli cells to produce new ampicillin-resistant colonies expressing their designed plasmids. Based on the orientation of the promoter, students then observe colonies that appear green (produce GFP), red (produce RFP), or blue (produce AmilCP blue). This gives them 3 distinct colonies to further assess using ImageJ software, freely available, to determine the level of protein expression.

The kit includes enough materials for 8 groups of 2 to 4 students. The activities typically take 3 to 6 class periods for completion. Note: Kit comes with the perishable materials included.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item ships with living or perishable materials (all   items). Please select a delivery date during checkout.

Components Qty Included?
Ampicillin, 10 mg/mL  4 Included
BsaI  1 Included
E. coli with Plasmid Containing Plac Promoter and RFP Gene  1 Included
E. coli with Plasmid Containing P5 Promoter and AmilCP Blue Gene  1 Included
T4 DNA Ligase  1 Included
10x Ligase Buffer  1 Included
MM294 E. coli Slant  1 Included
Mutant Oligonucleotide Pair (P5-33A top and bottom)  1 Included
Wild-Type Oligonucleotide Pair (P5 top and bottom)  1 Included
pClone Red Plasmid, 25 µg/µL  1 Included
pClone Blue Plasmid, 25 µg/µL  1 Included
10x Oligonucleotide Annealing Buffer 1 Included
Calcium Chloride, 50 mM, 3 mL 8 Included
Disposable Spreaders, Sterile 30 Included
Disposable Inoculating Loops, Sterile 30 Included
LB Agar, Sterile, 400 mL 4 Included
LB Media, 3 mL 8 Included
Petri Dishes 60 Included
Transformation Tubes, 15 mL 25 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Aluminum Foil Needed, Not Included
Beaker, 1 L Needed, Not Included
Computer Needed, Not Included
Digital Camera Needed, Not Included
Disinfectant (ethanol or household bleach solution) Needed, Not Included
Hot Plate or Bunsen Burner Needed, Not Included
Ice Needed, Not Included
ImageJ Software (free download) Needed, Not Included
Incubator, 37° C (optional) Needed, Not Included
Lab Marker Needed, Not Included
Microcentrifuge Needed, Not Included
Microcentrifuge Tubes, 0.5 mL Needed, Not Included
Micropipettors Needed, Not Included
Micropipette Tips, Sterile Needed, Not Included
Parafilm® Needed, Not Included
PCR Tubes, 0.5 or 2.0 mL Needed, Not Included
Programmable Thermal Cycler Needed, Not Included
Thermometer Needed, Not Included
Tongs Needed, Not Included
Tube Racks, for 0.5-mL Tubes Needed, Not Included
Tube Racks, for 15-mL Tubes Needed, Not Included
UV Transilluminator Needed, Not Included
White-Light Transilluminator (optional) Needed, Not Included
Water Needed, Not Included
Water Bath, 42° C Needed, Not Included
Wire Inoculating Loop Needed, Not Included
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