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Teach your students how to purify a green fluorescent jellyfish protein produced in E. coli!
Bringing genetic engineering and protein purification into your classroom has never been simpler. Module 2 is designed to allow students to purify GFP from the E. coli cells they successfully engineered in a previous transformation experiment. This lab activity requires prior completion of Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).
Intermediate - Requires some background knowledge.
This kit module is great for teaching:
This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 2 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).
In this activity students take their successfully transformed bacteria and use HIC resin to conduct a batch purification technique. This purification process doesn’t require the time or expense typically associated with column-based purification protocols; instead, it produces quick results in 1 class period (40 minutes).
Note: Kit ships with the perishable materials included.
|Ampicillin, 4 mL (10mg/mL)||1||Included|
|HIC Bead Resin, 300 µL||8||Included|
|Equilibration Buffer, 10 mL||1||Included|
|Lysis Buffer, 600 µL||8||Included|
|Binding Buffer, 400 µL||8||Included|
|Wash Buffer, 600 µL||8||Included|
|TE Buffer, 600 µL||8||Included|
|Luria Broth (LB), 20 mL||1||Included|
|Microcentrifuge Tubes, 1.5 mL||48||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|+pGREEN GFP Plate (from Module 1)||1||Needed, Not Included|
|Shaking Water Bath or Incubator||1||Needed, Not Included|
|Pipets, 100-1,000 µL||Needed, Not Included|
|Pipet Tips||Needed, Not Included|
|Microcentrifuge||1||Needed, Not Included|
|Permanent Markers||Needed, Not Included|
|Ice Buckets||Needed, Not Included|
|UV Light Source||Needed, Not Included|
|Waste Containers||Needed, Not Included|
|Vortex (optional)||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.