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Bringing genetic engineering and protein purification into your classroom has never been simpler. Module 3 is designed to allow students to conduct electrophoretic analysis of their purified GFP protein to confirm successful isolation. This lab activity requires prior completion of Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), and Module 2 (purification, item #211072 or #211072P).
Advanced - For more experienced classes; requires some technical skill.
This kit module is great for teaching:
This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 3 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), and Module 2 (purification, item #211072 or #211072P).
In this activity students take their successfully purified GFP and run a polyacrylamide gel to determine the results of their purification technique. Students analyze the band of purified GFP, approximately 27kDa, and compare it to the banding pattern of unpurified cell lysate to determine the effectiveness of their purification process. This activity typically takes 90 minutes for students to prepare, load, and run their gels followed by a COOMASSIE® blue staining process.
Note: Kit ships with the perishable materials included. Refrigerate polyacrylamide gels and protein marker until use.
|Protein Marker, 30 µL||1||Included|
|Tris-Glycine-SDS Buffer, 500 mL||1||Included|
|4x Loading Dye, 100 µL||4||Included|
|COOMASSIE® Blue Protein Staining Solution, 500 mL||1||Included|
|Microcentrifuge Tubes, 1.5 mL||24||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Purified GFP and Cell Lysate (from Module 2)||Needed, Not Included|
|Hot Plate or Heating Block, 95° C||Needed, Not Included|
|Permanent Markers||Needed, Not Included|
|Pipets, 2-20 µL||Needed, Not Included|
|Pipet Tips||Needed, Not Included|
|Protein Gel Electrophoresis Apparatus and Power Supply||Needed, Not Included|
|Distilled or Deionized Water||Needed, Not Included|
|Gloves||Needed, Not Included|
|Ice Buckets||Needed, Not Included|
|White Light Transilluminator (optional)||Needed, Not Included|
|Digital Camera (optional)||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.