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In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).
IntermediateEasy to perform; requires some background knowledge.
In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. For more advanced students, a discussion of nonsense mutations and nonsense suppressing mutations is included with the teacher background information. Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return coupon to request delivery of perishable materials. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).
|E. coli BE Slant Culture||1||Included|
|E. coli CR63 Slant Culture||1||Included|
|Transducing Lysate, 300 µL||1||Included|
|Ampicillin, 10 mg/mL, 4 mL||2||Included|
|Glass Beads, 1 oz||2||Included|
|Luria Broth Agar, 400 mL||4||Included|
|Luria Broth, 50 mL||3||Included|
|Sterile Pipets, 1 mL||4||Included|
|Sterile Pipets, 10 mL||2||Included|
|Sterile Culture Tubes||100||Included|
|Sterile Petri Plates||65||Included|
|Sterile Transfer Pipets||24||Included|
|Digital Resource Instruction Card||1||Included|
|Teacher’s Manual and Reproducible Student Guide||1||Included|
|Micropipets (10 µL and 50 µL)||Needed, Not Included|
|Sterile Tips for Micropipets||Needed, Not Included|
|Sterile Microcentrifuge Tubes||Needed, Not Included|
|Pipet Bulb or Automatic Pipet||Needed, Not Included|
|Large Waste Beakers||8||Needed, Not Included|
|Large Glass Bead Collection Beakers||8||Needed, Not Included|
|Boiling Water Bath (or microwave)||1||Needed, Not Included|
|Test Tube Racks||Needed, Not Included|
|Permanent Marker||Needed, Not Included|
|Bunsen Burner||Needed, Not Included|
|Incubator, 37° C (optional)||Needed, Not Included|
|Water Bath, 55° C (optional)||Needed, Not Included|