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Transduction of an Antibiotic-Resistance Gene Kit (with prepaid coupon)

Item # 211128

In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).

Price

$169.95

In stock and available to ship.

Intermediate—Easy to perform; requires some background knowledge.

In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. For more advanced students, a discussion of nonsense mutations and nonsense suppressing mutations is included with the teacher background information. Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return coupon to request delivery of perishable materials. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli BE Slant Culture 1 Included
E. coli CR63 Slant Culture 1 Included
Transducing Lysate, 300 µL 1 Included
Ampicillin, 10 mg/mL, 4 mL 2 Included
Glass Beads, 1 oz 2 Included
Luria Broth Agar, 400 mL 4 Included
Luria Broth, 50 mL 3 Included
Sterile Pipets, 1 mL 4 Included
Sterile Pipets, 10 mL 2 Included
Sterile Culture Tubes 100 Included
Inoculating Loops 4 Included
Sterile Petri Plates 65 Included
Sterile Transfer Pipets 24 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual and Reproducible Student Guide 1 Included
Micropipets (10 µL and 50 µL) Needed, Not Included
Sterile Tips for Micropipets Needed, Not Included
Sterile Microcentrifuge Tubes Needed, Not Included
Pipet Bulb or Automatic Pipet Needed, Not Included
Large Waste Beakers 8 Needed, Not Included
Large Glass Bead Collection Beakers 8 Needed, Not Included
Boiling Water Bath (or microwave) 1 Needed, Not Included
Test Tube Racks Needed, Not Included
Permanent Marker Needed, Not Included
Bunsen Burner Needed, Not Included
Incubator, 37° C (optional) Needed, Not Included
Water Bath, 55° C (optional) Needed, Not Included
Carolina Biological Supply

© Carolina Biological Supply Company

2700 York Road, Burlington, NC 27215-3398 • 800.334.5551