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Explaining the importance of DNA repair systems in your classroom doesn’t always click with students. Help shine a light on this concept, literally, by having your students observe the impact of ultraviolet (UV) light on yeast strains. This kit uses 2 yeast strains, wild-type and a UV-sensitive mutant, with an inquiry-based approach to get your students talking about the importance of DNA repair systems and the impact of UV damage. Includes enough materials for 32 students.
A fresh inquiry update to our popular Sunscreen for Yeast - and People Too! Kit
Using a UV-sensitive mutant strain of the yeast Saccharomyces cerevisiae, students investigate the impact of UV light on living organisms. The S. cerevisiae mutant strain is extremely sensitive to UV light because it lacks an effective method for repairing damaged DNA. In the first activity, students determine the impact of UV light on 2 strains of yeast, wild-type and UV-sensitive. After understanding the true impact of UV light, students then have the opportunity to design their own inquiry investigation to determine how to best prevent UV damage to the UV-sensitive yeast strain. In the second activity, these investigations can have students testing various sunscreens, cloth samples, or myriad other options of their own choosing. This entire activity is accomplished in 3 to 4 class periods and will truly give you a great starting point for discussing DNA repair systems and the impact of UV light on the human body. This 8-Station Kit includes enough materials for 32 students working in groups of 4.
|Wild-Type Yeast Culture||1||Included|
|UV-Sensitive Yeast Culture||1||Included|
|Sterile Toothpicks, Box||8||Included|
|YED Media, 350 mL||4||Included|
|Culture Tubes, Sterile||24||Included|
|Transfer Pipets, 1 mL||40||Included|
|Sterile Distilled Water, 20 mL||8||Included|
|Glass Beads, 1 oz||1||Included|
|Teacher's Manual with Reproducible Student Guide||1||Included|
|Aluminum Foil||Needed, Not Included|
|Plastic Wrap||Needed, Not Included|
|Waste Container||Needed, Not Included|
|Ethanol or Bleach||Needed, Not Included|
|Various Types of Sunscreen (optional for inquiry)||Needed, Not Included|
|Various Types of Fabric (optional for inquiry)||Needed, Not Included|
|Incubator (optional)||Needed, Not Included|