Login or Register


Carolina STEM Challenge®: Biofuels Kit

Product Highlights
  • Carolina Kits 3D®—Labs that use phenomena to support NGSS and 3D instruction
  • Meets the following NGSS Performance Expectation(s): HS-LS2-3 & HS-ETS1-1
  • Includes FREE digital resources for teacher and student instructional support
(in stock)


Product Details
Explore the production of ethanol biofuel by investigating the effect of enzymes and temperature on the breakdown of starch. Next, design and build a reaction chamber that will allow the production of ethanol by yeast fermentation and also allow the carbon dioxide gas produced to be measured. This high school engineering design challenge includes enough materials for 8 lab groups. Carolina Kits 3D®—Lab activity that designs a solution for an engineering problem to support NGSS and 3-dimensional instruction.

Students study the use of enzymes and microbes to produce ethanol biofuel. They investigate the ability of enzymes to break down starch under different conditions, and they determine the best conditions for using enzymes in ethanol production. Student teams brainstorm, design, build, and test an apparatus to capture and measure gas production during fermentation. They enter their engineered apparatus into a classroom competition, which can be scored using the design challenge scoring rubric. A grading rubric is also provided, facilitating assessment of each design team’s results. Team presentations allow students the opportunity to reflect on their experience and demonstrate their learning in creative ways.

Time Requirement
Teacher prep, 45 minutes. Engineering challenge, approximately 5 class periods.

Digital Resources
Includes 1-year access to digital resources that support 3-dimensional instruction for NGSS. Digital resources may include a teacher’s manual and student guide, pre-lab activities and setup videos, phenomenon videos, simulations, and post-lab analysis and assessments.

Performance Expectations

Crosscutting Concepts
Influence of Science, Engineering, and Technology on Society and the Natural World

Disciplinary Core Ideas
LS2.B: Cycles of Matter and Energy Transfer in Ecosystems

Science and Engineering Practices
Constructing Explanations and Designing Solutions

Learning Objectives

  • Investigate the effects of temperature and enzymes on alcohol fermentation.
  • Explore the process of ethanol production for use as biofuel, a renewable energy resource.
  • Design and build an apparatus that measures gas production during fermentation.

Prerequisite Knowledge and Skills
Prior knowledge of renewable energy resources, alcohol fermentation, and environmental science are useful in this experiment.


What’s Included:
Needed But Not Included:
Return Policy:

This product is backed by our 100% Satisfaction Guarantee. If for any reason you are not satisfied with any item, you may return it for a replacement, a refund, or credit. Read more about our Return and Exchange Policy  »


Questions & Answers