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Carolina Investigations® for Use with AP® Chemistry: Using a Titration to Determine the Amount of Calcium in Milk Kit

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Determine the concentration of calcium in milk by complexometric titration. Teach this laboratory experience using either the guided activity or inquiry activity. In the guided activity, students prepare and standardize a solution of EDTA and use it to determine the concentration of calcium in milk. Both the standardization and sample titrations are done 3 times and the average of the values used in the calculations. The inquiry activity allows students to design a procedure to determine the concentration of calcium in milk and requires them to account for interfering substances such as magnesium and other transition metals. The inquiry activity provides an opportunity for students to present their experiment design and results, reinforcing the practice of communicating findings. Detailed preparation and procedure notes guide the teacher through leading a successful inquiry exercise, including a suggested rubric for assessing student performance.

Both activities include assessment questions that follow the format of the AP® Chemistry Exam free-response questions. In the assessment, students consider another complexometric titration, the titration of zinc in zinc lozenges with EDTA, and calculate the concentration of zinc in the lozenge based on data provided. They also are required to consider what to do if they forget to standardize their titrant before they titrate the zinc.

Provides the following AP® Chemistry experiences: design a procedure to collect data, analyze data, perform error analysis, write and balance chemical equations, use volumetric glassware, standardize a titrant, and perform multiple trials. Analyzing calcium in milk engages students and makes the concepts covered more interesting. Materials are sufficient for 30 students working in teams of 3. Digital teacher's manual, included FREE with kit purchase, is a 12-month eBook license to the Complexometric Titration of Calcium in Milk teacher's manual. You can also access the digital student guide for this kit for free at Carolina Science Online®.

AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, these products.


What’s Included:
  • 1 Disodium EDTA Dihydrate, 25 g
  • 1 Hydrochloric Acid, 6 M, 15 mL
  • 1 Ammonia, 8 M, 250 mL
  • 1 Ammonium Chloride, 20 g
  • 1 Calmagite Indicator, 0.1%, 30 mL
  • 1 Powdered Milk, 1 qt
  • 1 Calcium Chloride, 0.1 M, 120 mL
  • 1 Calcium Chloride Dihydrate, 10 g
  • 30 Graduated Transfer Pipets, 1.0 mL
  • 1 pH Paper, 1-14 pH, Roll
  • 1 Milk Nutrition Label
  • 1 Carolina Science Online® Resources (1-year subscription)
  • 1 Teacher's Manual with Reproducible Student Guide
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Needed But Not Included:
  • 10 Thin-Stemmed Funnels
  • 10 Ring Stands
  • 10 Buret Clamps
  • 10 Beaker, 500 mL (for waste)
  • 10 Erlenmeyer Flasks, 125 mL
  • 20 Beakers, 100 or 150 mL
  •  Film or Protective Cover (for bottles or flasks)
  •  Distilled Water
  •  Plastic Bottle, 1 L (for EDTA solution)
  •  Weigh Papers or Boats
  •  Stir Plate (with magnetic stir bar)
  •  Balances (accurate to 0.01 g)
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