We use cookies to provide you with a great user experience. By using our site, you accept our use of cookies. You can review our cookie and privacy policy here.
  • Service & Support

    Contact Us

    Our Customer Service team is available from 8am to 6:30pm, ET, Monday through Friday. Live chat is available from 8am to 5:30pm ET, Monday-Friday.

    Call:
    800.334.5551
    Fax:
    800.222.7112
    Email:
    Email Customer Service
     

    International Sales & Service

    We serve educators in more than 170 countries worldwide. Create a quote request on our website or contact our International Sales Team.

    International Ordering
  • Shopping
    Lists

    Login or register now to maximize your savings and access profile information, order history, tracking, shopping lists, and more.

  • Quick
    Order
  • My Cart
    0

    My Cart

    Your Shopping Cart is currently empty. Use Quick Order or Search to quickly add items to your order!

Exploring Bacteriophages: Host Specificity and Plaque Morphology Kit (with voucher)

$112.75
(in stock)

Description

Students will use the double-agar layer technique to plate 2 different bacteriophages, ΦX174 and T2, on 2 different host bacteria. The next day they will count and describe the morphology of the resulting plaques. Students will observe that ΦX174 is only able to infect one of the host bacteria and that the plaques formed by the 2 different phages are very different in size.

This kit contains enough material for the experiment to be performed 1 time by either 1 group of students or 2 groups of students sharing the work. The digital resources that come with this kit include a video demonstrating the double-agar layer technique. The hands-on portion of the lab can be completed in one 50-minute session followed by a 15-minute session the next day. The prelab can be completed in 30 minutes and the analysis and assessment can be completed in 45 minutes.

This kit replaces and extends the Plaque Demonstration Kit B (items #124315 and #124315P).

Objectives

  • Use knowledge of the bacteriophage lytic life cycle to answer questions about the effect of bacteriophages on the bacterial host and its environment
  • Describe the morphology of plaques caused by different bacteriophages
  • Observe the concept of host specificity and thus be introduced to the importance of host genetics to a pathogen
  • Practice sterile technique and how to perform serial dilutions
  • Discuss the importance of controls
  • Draw conclusions from observed data
  • Demonstrate the understanding that patterns seen in simpler biological systems can help in the understanding of the biological systems of more complex organisms

Prerequisite Knowledge and Skills

  • Familiarity with sterile technique
  • Basic knowledge of bacteria, DNA, and proteins

Includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishables. Note: Refrigerate coliphage until use; do not freeze. Keep bacteria culture and agar at room temperature.

For Educational Use Only. All orders are subject to Carolina’s Terms and Conditions

Specifications

What’s Included:
Needed But Not Included:
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

Reviews

Questions & Answers

Loading...