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Antibiotic Sensitivity Demonstration Kit (with prepaid coupon)

Item # 154747 Exclusive This product is exclusive to Carolina Biological Supply. With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.

Product Highlights

  • Gather laboratory evidence of bacterial antibiotic sensitivity and construct an explanation for how antibiotic-resistant bacteria evolve.
  • High school teacher-led, student-engaged demonstration with enough materials for one teacher demonstration, a single student, or one lab group.
  • Carolina Kits 3D®—Labs that use phenomena to support NGSS and 3-dimensional instruction.
  • Kit includes a prepaid coupon to request delivery of perishable materials later at your convenience.



In stock and available to ship.

During the pre-lab, students investigate the phenomenon of the development of methicillin-resistant Staphylococcus aureus (MRSA) to analyze a timeline and correlate the introduction of antibiotics to the discovery of resistant bacteria. In the investigation, students test the inhibiting effects of antibiotics on 2 species of bacteria and produce evidence to address the driving question, "Why do we need to have so many different types of antibiotics?"

Note: Kit includes a prepaid coupon to request delivery of perishable materials later at your convenience. Contact Carolina to request delivery of perishables. Keep bacteria cultures at room temperature; do not incubate or refrigerate. Refrigerate antibiotic disks until use; do not freeze.

Time Requirement
Total, 180 minutes over 3 to 4 days. Teacher prep, 40 minutes; pre-lab, 30 minutes. Investigation, 20 to 30 minutes during 3 consecutive class periods (3 consecutive days). Assessment and discussion, 30 minutes. Note: The cultures can be refrigerated if students cannot check them for 3 consecutive days.

Digital Resources
Includes 1-year access to digital resources that support 3-dimensional instruction for NGSS. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, phenomenon videos, simulations, and post-lab analysis and assessments.

Performance Expectation(s)

Crosscutting Concepts
Cause and Effect

Disciplinary Core Ideas
LS4.B: Natural Selection

Science and Engineering Practices
Constructing Explanations and Designing Solutions

Learning Objectives

  • Understand that the trait of antibiotic sensitivity varies both within and between species of bacteria.
  • Observe, measure, and compare the effects of antibiotics on 2 bacteria species.
  • Describe how the collected data provide evidence of sensitivity or resistance to the antibiotic.
  • Use evidence from a variety of sources to explain why so many antibiotics are needed.

Prerequisite Knowledge and Skills
Students should know the basic characteristics of bacteria and the basic function of antibiotics. They should also have a general understanding of traits and heritability.

For Educational Use Only. All orders are subject to Carolina’s Terms and Conditions

  • This item is only available from Carolina Biological Supply Company.

Product Families Featuring This Item:


This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Bacillus cereus Broth Culture 1 Included
Escherichia coli Broth Culture 1 Included
Ampicillin Antibiotic Disks 2 Included
Chloramphenicol Antibiotic Disks 2 Included
Gentamycin Antibiotic Disks 2 Included
Penicillin Antibiotic Disks 2 Included
Streptomycin Antibiotic Disks 2 Included
Tetracycline Antibiotic Disks 2 Included
Nutrient Agar, 20-mL vials 2 Included
Sterile Petri Dishes 2 Included
White Control Disk 1 Included
Forceps 1 Included
Autoclave Disposal Bag 1 Included
Techniques for Studying Bacteria and Fungi Manual 1 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Metric Rulers 1 Needed, Not Included
Labeling Marker 1 Needed, Not Included
Bunsen Burner or Alcohol Burner 1 Needed, Not Included
Water Bath with Thermometer 1 Needed, Not Included
Test Tube Rack 1 Needed, Not Included
Heat-Resistant Gloves, Pair 1 Needed, Not Included
Paper Towels Needed, Not Included
Goggles Needed, Not Included
Lab Apron Needed, Not Included
Gloves Needed, Not Included
Matches Needed, Not Included
Cellophane Tape Needed, Not Included
Disinfectant (70% ethanol or bleach) Needed, Not Included
Incubator (optional) Needed, Not Included
Autoclave (optional) Needed, Not Included

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