Exploring the CRISPR-Cas Defense System Kit (with prepaid coupon) | Carolina.com

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Exploring the CRISPR-Cas Defense System Kit (with prepaid coupon)

Item # 211772 New Online Only
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Intermediate - Easy to perform; requires some basic training in microbiology.
Students conduct experiments and analyze data over the course of this 5-day lab to understand the relationship between a phage called Andhra and its staphylococcus host. Their goal is to answer the driving question, How can we use evidence to model phage-host interactions and therefore explain the impact of a CRISPR-Cas system on bacterial survival?

In stock and available to ship.

Intermediate - Easy to perform; requires some basic training in microbiology.
Bring new gene-editing technology into your classroom!
Your students walk in the footsteps of the scientists that first discovered this exciting gene-editing technology. Originally discovered as an integral part of the immune system of bacteria, you and your class explore the CRISPR-Cas system and its role in defending the host bacteria from viral bacteriophages. Bacteriophages outnumber bacteria 10:1 and have evolved an intricate immune system to combat invading phages. One such system, the CRISPR-Cas, uses small pieces of RNA to detect phages with matching DNA sequences, and destroys them.

Over the course of this 5-day lab, students conduct experiments and analyze data to understand the relationship between a phage called Andhra and its staphylococcus host and answer the driving question, How can we use evidence to model phage-host interactions and therefore explain the impact of a CRISPR-Cas system on bacterial survival? Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
S. epidermidis LM1680 Culture (3 x 1-mL aliquots) 1 Included
S. epidermidis Strain A Culture (3 x 1-mL aliquots)  1 Included
S. epidermidis Strain B Culture (3 x 1-mL aliquots)  1 Included
Phage Lysate 6 1 Included
Sterile Tryptic Soy Broth (TSB) 1 Included
Tryptic Soy Agar (TSA), 125 mL 1 Included
Sterile Petri Plates, 100 mm 40 Included
Heart Infusion Agar, 4 mL 1 Included
Tryptic Soy Broth (TSB), 4 mL 1 Included
Sterile Individually Wrapped Transfer Pipettes 80 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Microbiological Plate Incubator, 37° C 1 Needed, Not Included
Refrigerator, 4° C 1 Needed, Not Included
Boiling Water Bath 1 Needed, Not Included
Autoclave 1 Needed, Not Included
Disposable Gloves (2 pairs per student, minimum) Needed, Not Included
Lab Coats (1 per student) Needed, Not Included
Safety Glasses (1 pair per student) Needed, Not Included
Test Tube Racks (for 13 x 100-mm tubes) 6 Needed, Not Included
Heat-Resistant Mitt 1 Needed, Not Included
Rubbing Alcohol (70%), Household Bleach Solution (10%), or Lysol® Needed, Not Included
Paper Towels Needed, Not Included
Permanent Markers 6 Needed, Not Included
Tupperware® Tray (for bacterial plate transport/storage) 2 Needed, Not Included
Tupperware® Tray (for bacterial plate transport/storage) 2 Needed, Not Included
Biohazard Disposal Bags 2 Needed, Not Included
Biohazard Disposal Bags 2 Needed, Not Included
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