P51™ Enzyme Lab: β-Gal Glow™ | Carolina.com

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P51™ Enzyme Lab: β-Gal Glow™

Item # 216323 New Online Only
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Intermediate—Easy to perform; requires some background knowledge.
Students investigate enzyme activity and the conditions that affect it. In this lab, a substrate fluoresces under blue light when sugar molecules are removed by the enzyme beta-galactosidase. Observing how brightly the solution fluoresces over time allows students to observe the reaction progress, and to quantify the effect of different factors on enzyme efficiency.

Price

$89.00

Ships from the manufacturer and will arrive on or about 6/11/19

Intermediate—Easy to perform; requires some background knowledge.
This lab enables students to investigate enzyme activity and the conditions that affect it. Under optimal conditions enzymes catalyze, or speed the rate of, chemical reactions. In this lab, a substrate that is not fluorescent under basal conditions will fluoresce under blue light when sugar molecules are removed through hydrolysis by the enzyme beta-galactosidase. Observing how brightly the solution fluoresces over time allows students to observe the reaction progress, and to quantify the effect of different factors on enzyme efficiency.

The lab is structured as 4 inquiry-based investigations. In each investigation, students explore the effect of the following factors on enzyme reaction rate:

  • pH
  • Temperature
  • Enzyme and substrate concentration
  • Competitive inhibition

Enough reagents are provided for 8 lab groups (32 students) to each pursue at least 2 of the investigations. This activity is compatible with blueGel™ (item #213900), P51™ Molecular Fluorescence Viewer (item #216321), or other blue light transilluminator systems (not included) and gives students practice with micropipets and using fluorescence to detect enzyme activity. It can be completed in one 45-minute class period or be extended to two 45-minute periods. Note: Reagents require refrigerated storage and have a shelf life of 6 months when stored at 4° C.

  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is only available at Carolina.com.
 
Components Qty Included?
Substrate for Enzymatic Reaction 1 Included
Purified Beta-Galactosidase Enzyme Solution 1 Included
Dilution Buffers 2 Included
Inhibitor Solutions 2 Included
Alkaline Medium 1 Included
Fluorescent Solution for Standard Curve 1 Included
Student's Guide (download from www.minipcr.com) Needed, Not Included
Instructor's Guide (download from www.minipcr.com) Needed, Not Included
Blue Light Illuminator 1 Needed, Not Included
miniPCR® Thermal Cycler (suggested for temperature investigations) 1 Needed, Not Included
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