We use cookies to provide you with a great user experience. By using our site, you accept our use of cookies. You can review our cookie and privacy policy here.
  • Service & Support

    Contact Us

    Our Customer Service team is available from 8am to 6:30pm, ET, Monday through Friday. Live chat is available from 8am to 5:30pm ET, Monday-Friday.

    Call:
    800.334.5551
    Fax:
    800.222.7112
    Email:
    Email Customer Service
     

    International Sales & Service

    We serve educators in more than 170 countries worldwide. Create a quote request on our website or contact our International Sales Team.

    International Ordering
  • Shopping
    Lists

    Login or register now to maximize your savings and access profile information, order history, tracking, shopping lists, and more.

  • Quick
    Order
  • My Cart
    0

    My Cart

    Your Shopping Cart is currently empty. Use Quick Order or Search to quickly add items to your order!

CRISPR in a Box™ Kit

$679.00
(ships from manufacturer, est arrival 10/14/22)

Description

An AUTHENTIC Gene Editing Experience
Discover CRISPR technology the way it was meant to be with this truly unique, hands-on CRISPR gene editing kit. As your students explore Nobel Prize-winning technology, they walk in the footsteps of science pioneers and use the same techniques that scientists across the world have used to solve the global problems of tomorrow.

Designed to be safe and approachable, the kit activities break down each portion of the gene editing reaction into fundamental steps, offering teachable moments along the way. Students will:

  • Understand how CRISPR interacts with DNA
  • Carry out gene editing reactions in a cell-free environment
  • Visualize all gene editing products from a single reaction
  • Develop a fundamental understanding of the degree of mutagenesis and precise or error-prone gene editing outcomes
  • Explore the effects of different repair pathways acting simultaneously (HDR, NHEJ, MMEJ, etc.)
  • Study gene knockout and knock-in strategies in one reaction

Kit includes background information, experimental overview, timelines, expected results, and more, plus enough materials for 10 reactions.

Specifications

What’s Included:
  • 1 3.1 Buffer, 10x, 25 µL
  • 1 Carbenicillin, 600 µL
  • 1 Cas12a Nuclease, 2 µL
  • 1 Cell-Free Extract, 50 µL
  • 1 crRNA1364, 10 µL
  • 1 HDR-NS Oligonucleotide, 10 µL
  • 1 IPTG, 700 µL
  • 1 pUC19 Plasmid, 50 µL
  • 1 Ligase, 12 µL
  • 1 X-gal, 700 µL
  • 1 In Vitro Reaction Buffer, 10x, Lyophilized
  • 1 Cas Buffer, 100 µL
  • 1 RNA Buffer, 100 µL
  • 1 C&C Binding Buffer, 1.3 mL
  • 1 C&C Wash Buffer, 11 mL
  • 1 LB Agar Powder, 18 g
  • 1 Water, UltraPure, 2 mL
  • 1 Miniprep P1, 6.3 mL
  • 2 LB Broth Capsules
  • 1 Miniprep Binding Buffer, 6.9 mL
  • 1 Miniprep P2, 6.3 mL
  • 1 Miniprep P3, 6.3 mL
  • 1 Miniprep Wash 1, 19.8 mL
  • 1 Miniprep Wash 2, 24 mL
  • 1 Competent Buffer, 2x, 83 µL
  • 1 Wash Buffer, 2x, 83 µL
  • 1 DH5α E. coli, Lyophilized
  • 1 Dilution Buffer, 1.7 mL
  • 1 ZymoBroth™, 18 mL
  • 1 Digestion Buffer, 10x, 52 µL
  • NotI Restriction Enzyme, 26 µL
  • XmnI Restriction Enzyme, 26 µL
  • 1 Diamond Ready-to-Use DNA Ladder (0.1–10 Kbp), 11 µL
  • 1 TAE Buffer, 50x, 22.5 mL
  • 1 Sample Loading Dye, 6x, 80 µL
  • 1 Agarose, 1.1 g
  • 1 SYBR™ Safe DNA Gel Stain, 11 µL
  • 20 Tubes, 0.2 mL
  • 190 Tubes, 1.5-mL
  • 20 C&C Collection Tubes
  • 20 C&C Spin Columns
  • 20 Inoculating Loops
  • 20 Inoculation Spreaders
  • 20 Miniprep Collection Tubes
  • 20 Miniprep Spin Columns
  • 20 Petri Dishes
  • 22 Round Bottom Tubes
  • 1 Instruction Manual
Needed But Not Included:
  • 5 Micropipettes, Adjustable-Volume, 2–20 µL
  • 5 Micropipettes, Adjustable-Volume, 20–200 µL
  • 5 Micropipettes, Adjustable-Volume, 200–1,000 µL
  • 5 Micropipette Tips, 20 µL, Box
  • 5 Micropipette Tips, 200, µL, Box
  • 5 Micropipette Tips, 1,000 µL, Box
  • 1 Bottle, Autoclavable, with Cap, 250 mL
  • 1 Bottle, Autoclavable, with Cap, 1 L
  • 1 Autoclave or Microwave Oven
  • 1 Centrifuge, Benchtop
  • 1 Transilluminator, Blue Light or UV
  • 2 Water, Deionized, Liter
  • 1 Erlenmeyer Flask, 125 mL
  • 1 Erlenmeyer Flask, 250 mL
  • 100 Gloves, Disposable, Small
  • 100 Gloves, Disposable, Medium
  • 100 Gloves, Disposable, Large
  • 1 Graduated Cylinder, 100 mL
  • 1 Graduated Cylinder, 500 mL
  • 1 Graduated Cylinder, 1 L
  • 1 Horizontal Gel Electrophoresis Chamber with Gel Casting Tray and Comb
  • 5 Pens, Permanent Marking
  • 1 Power Supply
  • 1 Shaking Incubator
  • 1 Stationary Incubator
  • 1 Spectrophotometer and Associated Materials
  • 1 Temperature-Controlled Dry Bath or Water Bath
  • 5 Tube Racks
  • 5 Wet Ice and Containers (e.g., ice bucket)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

Reviews

Questions & Answers

Loading...