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Inducing RNAi by Feeding Kit

3 Items
Inducing RNAi by Feeding Kit is rated 4.3 out of 5 by 4.
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$5.15 - $188.00 View Details

Advanced – For experienced high school and college classes; requires some technical skill.
Demonstrate to your students the power of silencing a single gene. RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading its mRNA. In this kit, students use this Nobel Prize-winning technique to silence 2 different genes in the nonparasitic roundworm C. elegans, a model organism used for studying many aspects of biology.

 
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Advanced – For experienced high school and college classes; requires some technical skill.

  • Demonstrate to your students the power of silencing a single gene.
  • Teach about a powerful method for determining gene function.
  • Introduce your students to a model organism used for studying many aspects of biology, including development and gene function.
  • Engage in bioinformatics exercises exploring protein function and C. elegans and human gene relatedness.
In this lab, wild-type worms are fed lab strains of E. coli that express double-stranded RNA (dsRNA) corresponding to either of 2 target genes: BLIstered cuticle (bli-1) or DumPY (dpy-11). The ingestion of the dsRNA initiates the destruction of mRNA expressed from the target genes. Students then examine the phenotypes induced by silencing these genes. Silencing bli-1 leads to the formation of clear blisters in a worm's outer cuticle; silencing dpy-11 leads to a worm developing a short, fat body. In accompanying bioinformatics exercises, students explore the function of the protein encoded by the silenced gene and the relatedness of worm and human genes.

Note: Order the kit with the perishable materials included (item #211391P) or with a prepaid coupon to request perishables later at your convenience (item #211391). Because of the time-sensitive preparation and limited shelf life of the kit's perishable materials, please order a minimum of 2 weeks prior to the desired ship date. Plan on using the materials within a week of receiving them. Perishables ship only on Mondays, Tuesdays, and Wednesdays via standard overnight delivery.

 
 
 
 
Rated 4 out of 5 by from "I have never seen so much excitement over worms!" said my administrator observing my class. My high school Genetics class undertook this experiment to see RNAi in action. The experiment gives them experience in plating bacteria, growing C. elegans worms and "chunking" the worms. The most important outcome was actually seeing the different phenotypes that resulted from the RNAi process. Make sure your students know what to look for in the mutants. Teacher pre-practice with picking worms is helpful in guiding the students.
Date published: 2014-12-19
Rated 4 out of 5 by from Great for Biotech classes I use this kit in my H.S. Biotech classes (Juniors and Seniors). It is great to teach RNA interference in a hands-on way. It is equally valuable to have students gain experience working with a model organism (C. Elegans). Sometimes is difficult for students to identify the mutant phenotypes, and we have occasional mold contamination issues, but other than that, this is a great kit.
Date published: 2014-11-19
Rated 5 out of 5 by from Simple RNAi Lab Such a simple lab to perform to teach a relatively new concept! I highly recommend it!
Date published: 2013-10-25
Rated 4 out of 5 by from Good Starter Spaula I'm very happy with these micro spatulas. They work well and are a good length.
Date published: 2009-03-29
  • y_2018, m_10, d_15, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_FAM_211391, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_carolina
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 28ms
  • REVIEWS, PRODUCT