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  • Examining the RNAi Mechanism Kit (RNAi, Amplification, and Electrophoresis Kit with Ethidium Bromide; with Perishables)

Examining the RNAi Mechanism Kit (RNAi, Amplification, and Electrophoresis Kit with Ethidium Bromide; with Perishables)

Item # 211394P
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Students use RNAi to silence the dpy-13 gene in the non-parasitic roundworm, C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, DumPY 13 (dpy-13). The ingestion of the dsRNA from the bacteria initiates the destruction of mRNA expressed from the targeted dpy-13 gene. Comes with perishable materials.

Price

$379.00

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Overview

Examining the RNAi Mechanism Kit, RNAi, Amplification, and Electrophoresis Kit with Ethidium Bromide. RNA interference (RNAi) is a technique that allows you to silence expression of a chosen gene by degrading the gene's mRNA. Kit lets students use the Nobel Prize-winning technique to silence the dpy-13 gene in non-parasitic roundworm, C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, DumPY 13 (dpy-13). Ingestion of dsRNA from bacteria initiates destruction of mRNA expressed from targeted dpy-13 gene. Students examine the short, fat phenotype induced by silencing the dpy-13 gene, and then isolate DNA from the RNAi-induced dpy-13 worms, wild-type worms, and from dpy-13 worms with a deletion in the dpy-13 gene. They amplify the region of the dpy-13 gene with the mutation to see if the corresponding region in the wild-type worms and RNAi-induced dpy-13 worms has the same mutation. Deletion mutation creates a size difference in amplified DNA fragment, easily seen by gel electrophoresis. In bioinformatics exercises, they explore function of protein encoded by the silenced gene and relatedness of human and worm genes. Kit includes: 0.036g Isopropyl B-D-Thio-Galactopyranoside, 2 tubes cholesterol (5mg/mL, 300µL), 4 mL ampicillin, proteinase K (20mg/mL, 25µL), DNA marker (pBR322/BstN1), primer/loading dye mix, 4 plates dpy-13 C. elegans, 4 plates wild-type (N2) C. elegans, E. coli slants (1 OP50 and 1 dpy-13 RNAi feeding strain), 25 PCR beads (in 0.2-mL tubes), lysis buffer (0.5-mL tube), 1mL sterile distilled water, 6 sterile dropping pipets, 2 melt n’ pPour NGM lite media bottles (100mL and 300mL), 40 petri dishes (60mm), 3 disposable inoculating loops, 3 sterile culture tubes, 3 LB broth (3-mL vials), 5mL mineral oil, 8 glass tubes of sterile toothpicks, 8g agarose, 20x TBE (200 mL), 8 staining trays, ethidium bromide, and instructor's manual with reproducible student guide.

FREE 1-year access to digital resources that support NGSS instruction included.

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  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
  • This is a special order item that requires additional time for culturing and processing before it ships. Arrival timeframes are available during Checkout.

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What's Included

This item ships with living or perishable materials (all items). Please select a delivery date during Checkout.

Components Qty Included?
Ampicillin, 10 mg/mL, 4 mL   1 Included
Cholesterol, 5 mg/mL, 0.3 mL   2 Included
IPTG, 0.036 g   1 Included
Proteinase K, 20 mg/mL, 25 μL   1 Included
DNA Marker pBR322/BstN1, 200 μL   1 Included
dpy-13 (e458) Worms on NGM-lite Plates   4 Included
dpy-13 Primer/Loading Dye Mix, 700 μL   1 Included
E. coli RNA Feeding Strain (dpy-13) LB Amp/Tet Slant   1 Included
E. coli OP50 on LB Slant   1 Included
Wild-type Worms on NGM-lite Plates   4 Included
1x Lysis Buffer, 0.5 mL 1 Included
Sterile Bulb Pipets 6 Included
Sterile Culture Tubes, 15 mL 3 Included
Sterile Inoculating Loops 3 Included
LB Broth, 3 mL 3 Included
Mineral Oil, 5 mL 1 Included
Melt 'n Pour NGM-lite, 300 mL 1 Included
Melt 'n Pour NGM-lite, 100 mL 1 Included
Sterile Petri Dishes, 60 mm 40 Included
Ready-to-Go™ PCR Beads (0.2 mL tubes) 25 Included
Sterile Distilled Water, 1 mL 1 Included
Sterile Toothpicks 8 Included
Agarose, 6 g 1 Included
Gloves 4 Included
TBE Buffer, 200 mL 1 Included
Ethidium Bromide, 1 μg/mL 1 Included
Staining Trays 8 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Stereomicroscope Needed, Not included
Bunsen Burner Needed, Not included
Containers with Cracked or Crushed Ice Needed, Not included
Container with Liquid Nitrogen or Dry Ice Needed, Not included
Digital or Instant Camera (optional) Needed, Not included
Ethanol, 95% Needed, Not included
Gel Casting Trays and Combs Needed, Not included
Gel Electrophoresis Chamber Needed, Not included
Masking Tape Needed, Not included
Microcentrifuge Needed, Not included
Microcentrifuge Adaptors Needed, Not included
Micropipettor and Tips, 1-20 μL and 20-200 μL Needed, Not included
Microcentrifuge Tube Rack Needed, Not included
Mineral Oil Needed, Not included
PCR Tubes, 0.2 or 0.5 μL Needed, Not included
Permanent Markers, Black and Red Needed, Not included
Power Supply Needed, Not included
Steel Scalpel, Spatula, or Forceps Needed, Not included
Thermal Cycler Needed, Not included
UV or White Light Transilluminator Needed, Not included
Water Bath, 60°C (for agarose solution) Needed, Not included
Heavy-duty Plastic Bag Needed, Not included
Household Bleach Needed, Not included

Resources

How-To & Techniques

Living Organism Care Information

Sample Teacher Manuals

 

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DNA Learning Center

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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