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Examining the RNAi Mechanism Kit (RNAi, Amplification, and Electrophoresis Kit with Ethidium Bromide; with voucher)

$353.35

Description

Advanced - For experienced high school and college classes; requires some basic technical skill.

  • Demonstrate to your students the power of silencing a single gene
  • Teach about a powerful method for determining gene function
  • Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
  • Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness
RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. Examining the RNAi Mechanism kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).

Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishables.

FREE 1-year access to digital resources that support NGSS instruction included.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

Specifications

Shipping Information or Purchase Restrictions
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
What’s Included:
  • 1 Ampicillin, 10 mg/mL, 4 mL (voucher item)
  • 2 Cholesterol, 5 mg/mL, 0.3 mL (voucher item)
  • 1 IPTG, 0.036 g (voucher item)
  • 1 Proteinase K, 20 mg/mL, 25 μL (voucher item)
  • 1 DNA Marker pBR322/BstN1, 200 μL (voucher item)
  • dpy-13 (e458) Worms on NGM-lite Plates (voucher item)
  • dpy-13 Primer/Loading Dye Mix, 700 μL (voucher item)
  • E. coli RNA Feeding Strain (dpy-13) LB Amp/Tet Slant (voucher item)
  • E. coli OP50 on LB Slant (voucher item)
  • 4 Wild-type Worms on NGM-lite Plates (voucher item)
  • 1 1x Lysis Buffer, 0.5 mL
  • 6 Sterile Bulb Pipets
  • 3 Sterile Culture Tubes, 15 mL
  • 3 Sterile Inoculating Loops
  • 3 LB Broth, 3 mL
  • 1 Mineral Oil, 5 mL
  • 1 Melt 'n Pour NGM-lite, 300 mL
  • 1 Melt 'n Pour NGM-lite, 100 mL
  • 40 Sterile Petri Dishes, 60 mm
  • 25 Ready-to-Go™ PCR Beads (0.2 mL tubes)
  • 1 Sterile Distilled Water, 1 mL
  • 8 Sterile Toothpicks
  • 1 Agarose, 6 g
  • 4 Gloves
  • 1 TBE Buffer, 200 mL
  • 1 Ethidium Bromide, 1 μg/mL
  • 8 Staining Trays
  • 1 Teacher's Manual and Reproducible Student Guide
Needed But Not Included:
  •  Document Camera or Overhead Projector
  •  Stereomicroscope
  •  Bunsen Burner
  •  Containers with Cracked or Crushed Ice
  •  Container with Liquid Nitrogen or Dry Ice
  •  Digital or Instant Camera (optional)
  •  Ethanol, 95%
  •  Gel Casting Trays and Combs
  •  Gel Electrophoresis Chamber
  •  Masking Tape
  •  Microcentrifuge
  •  Microcentrifuge Adaptors
  •  Micropipettor and Tips, 1-20 μL and 20-200 μL
  •  Microcentrifuge Tube Rack
  •  Mineral Oil
  •  PCR Tubes, 0.2 or 0.5 μL
  •  Permanent Markers, Black and Red
  •  Power Supply
  •  Steel Scalpel, Spatula, or Forceps
  •  Thermal Cycler
  •  UV or White Light Transilluminator
  •  Water Bath, 60°C (for agarose solution)
  •  Heavy-duty Plastic Bag
  •  Household Bleach
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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