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Examining the RNAi Mechanism

$317.30 - $353.35

Demonstrate the power of silencing a single gene. RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. This kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the non-parasitic round worm, C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).

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Description

  • Demonstrate to your students the power of silencing a single gene
  • Teach about a powerful method for determining gene function
  • Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
  • Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness
RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. The Examining the RNAi Mechanism kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the non-parasitic round worm, C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).

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