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Carolina EcoKits®: Coliform Contamination

Item # 187220 New Online Only Exclusive
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Grades 9–12. Students investigate possible water source contamination by coliform bacteria. Using sterile technique, they collect water samples and plate them on selective media to differentiate between general coliform, Escherichia coli, and noncoliform bacteria colonies. After 24 to 48 hr, results are analyzed. In the second investigation, students analyze samples and propose design solutions to reduce the environmental impacts of human activities linked to bacterial contamination of water.

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Grades 9–12. Students investigate possible water source contamination by coliform bacteria. Using sterile technique, they collect local water samples from approved locations and then plate them on selective media. The media allows students to differentiate between general coliform, Escherichia coli, and noncoliform bacteria colonies based on color. Students analyze the colonies 24 to 48 hr after plating. Based on the number of coliform and E. coli cultures, as well as any dilution factor, students determine if their samples meet standards for different water uses in their area.

In the second inquiry-based investigation, students analyze water samples from a fictional municipality for the presence of coliform and E. coli cultures. Then, based on activity results and inferred sources of possible contamination, students propose design solutions to reduce the environmental impacts of human activities linked to the water’s bacterial contamination. This kit helps address Next Generation Science Standard* HS-LS2-7, includes 1-year access to digital resources, and accommodates up to 10 groups of students.

Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.

*Next Generation Science Standards® is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards were involved in the production of this product, and do not endorse it.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is only available at Carolina.com.
 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Escherichia coli B MicroKwik Culture® 1 Included
Enterobacter aerogenes MicroKwik Culture® 1 Included
Dehydrated Chromogenic Medium, 5.2 g 1 Included
Sterile Collection Tubes 30 Included
Sterile Bulb Pipets 60 Included
Sterile Petri Dishes, 60 x 15 mm 20 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Permanent Markers 10 Needed, Not Included
Small Resealable Plastic Bags (at least 7" W) 40 Needed, Not Included
Lab Thermometer 1 Needed, Not Included
Bleach Needed, Not Included
Ethanol or Isopropanol Needed, Not Included
Water Samples (collected by students) Needed, Not Included
Sterile Distilled Water Needed, Not Included
Tubes for Dilution (if necessary) Needed, Not Included
Parafilm® Needed, Not Included
Paper Towels Needed, Not Included
Soap Needed, Not Included
Erlenmeyer Flask, 250 mL Needed, Not Included
Hot Plate Needed, Not Included
Incubator (optional) Needed, Not Included
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