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Succession in a Hay Infusion Kit

Item # 187224 Exclusive This product is exclusive to Carolina Biological Supply. With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.

Product Highlights

  • Gathering evidence over time, students observe and describe patterns in the relationships among populations within a hay infusion serving as a model pond.
  • High school laboratory investigation with enough materials for 8 lab groups.
  • Carolina Kits 3D®—Labs that use phenomena to support NGSS and 3-dimensional instruction.
  • Kit includes a prepaid coupon to request delivery of perishable materials later at your convenience.



In stock and available to ship.

Students explore the phenomenon of how ecosystems change over time and how these changes follow a general pattern called succession. During the experiment, students set up a mini pond using a hay infusion, protist cultures, and algae cultures to model a pond ecosystem. Student groups make observations and take samples of assigned portions of the pond, collecting evidence about changes in abiotic and biotic factors. Groups also create microscale pond models from test tubes—a control microscale pond, which mimics the features of the larger class pond, and a microscale pond with change to 1 or more parameters—to determine how changes would impact the ecosystem. Student observations and data collection take place over the course of 4 to 6 weeks as students strive to answer the driving question, “How do biological communities in a model pond ecosystem change over time?”

Time Requirement
Teacher prep, approximately 1 class period. Set up, pre-lab, investigation, and assessment, approximately 7 to 9 class periods over the course of 4 to 6 weeks.

Digital Resources
Includes 1-year access to digital resources that support 3-dimensional instruction for NGSS. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, phenomenon videos, simulations, and post-lab analysis and assessments.

Performance Expectations

Crosscutting Concepts
Stability and Change

Disciplinary Core Ideas
LS2.C: Ecosystem Dynamics, Functioning, and Resilience

Science and Engineering Practices
Developing and Using Models

Learning Objectives

  • Identify and draw microorganisms observed in a hay infusion.
  • Identify and describe changes observed in biotic and abiotic factors in a micro-ecosystem.
  • Explain ecological succession in a micro-ecosystem on the basis of patterns observed in the relationships in such a community over time.

Prerequisite Knowledge and Skills
Students should be familiar with terms that describe the structure and components of an ecosystem (biotic and abiotic factors, food chain, food web, species, population, habitat, trophic level, niche, and ecosystem). They should also know how to operate a microscope.

  • This item is only available from Carolina Biological Supply Company.
  • Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Protozoan Mixture  2 Included
Algae Mixture  2 Included
Alga-Gro® Freshwater Medium, Vial  2 Included
Soil, Bag 1 Included
Timothy Hay, Bag 1 Included
Cheesecloth, 5 yd 1 Included
Culture Tubes (20 x 150 mm) 20 Included
Pipets 400 Included
Plastic Depression Slides 16 Included
Plastic Tank, 1 gal 1 Included
Protoslo® Quieting Solution, 15-mL Bottle 1 Included
pH Paper (pH 4.5–7.5), Roll 1 Included
Wheat Seed, 1 Tablespoon 1 Included
Rubber Stoppers, 1-Hole 20 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Light Microscope (1 per student group) Needed, Not Included
Aluminum Foil (optional, see procedure) Needed, Not Included
Springwater (approximately 8 L) Needed, Not Included
Colored Pencils, Class Set (optional item for prelab) Needed, Not Included
Wax Pencil or Marker (1 per student group) Needed, Not Included
Labeling Tape (masking tape) Needed, Not Included
Graduated Cylinder (1 per student group) Needed, Not Included
Plastic Spoons or Scoopulas (1 per student group) Needed, Not Included
Ruler Needed, Not Included
Culture Tube or Test Tube Rack (1 per student group) Needed, Not Included
Waste Beaker or Cup (1 per student group) Needed, Not Included
Wash Bottle 1 Needed, Not Included
Paper Towels Needed, Not Included
Scissors Needed, Not Included
Electronic Scale or Balance Needed, Not Included