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Oil Spill Bioremediation Kit

Item # 181324 Exclusive This product is exclusive to Carolina Biological Supply. With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.

Product Highlights

  • Conduct and analyze a controlled oil biodegradation experiment, use observations to attempt to improve the simulation, and then explore data from the Deepwater Horizon oil spill.
  • High school laboratory investigation with enough materials for 8 lab groups.
  • Carolina Kits 3D®—Labs that use phenomena to support NGSS and 3-dimensional instruction.



This item will ship on or about 8/5/21

The role of bioremediation in the Deepwater Horizon oil spill (DHOS) serves as the investigative phenomenon as students conduct and analyze a controlled biodegradation experiment using drain cleaner microbes, corn oil, and a chemical indicator. Once students make sense of how the experiment serves as an oil spill bioremediation simulation, they ask questions and design their own experiments with biodegradation to see if they can make the simulation even more realistic. Next, they present their claims, evidence, reasoning, and arguments to their classmates. The investigation culminates in an assessment that has students interpret data from the DHOS, consider the role of bioremediation in oil spill cleanup, and summarize oil spill impacts on threatened and endangered species. Throughout the investigation students strive to understand the driving question, “How can microbes be used to clean up an oil spill?”

Time Requirement
Teacher prep, 20 minutes. Pre-lab, investigation, and assessment, approximately 6 class periods.

Digital Resources
Includes 1-year access to digital resources that support 3-dimensional instruction for NGSS. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, phenomenon videos, simulations, and post-lab analysis and assessments.

Performance Expectations

Crosscutting Concepts
Cause and Effect

Disciplinary Core Ideas
LS4.D: Biodiversity in Humans

Science and Engineering Practices
Asking Questions and Defining Problems
Engaging in Argument from Evidence

Learning Objectives

  • Observe the breakdown of corn oil by oil-degrading microbes and learn how microbes can be used in the bioremediation of marine oil spills.
  • Design and conduct a biodegradation experiment to answer a question posed after the pre-lab activity.
  • Consider the impact that bioremediation had on the DHOS. Describe how biodiversity was affected by the DHOS and recommend conservation efforts for endangered species.

Prerequisite Knowledge and Skills
Students should know what a septic system is and the definition of biodegradable. They should be familiar with bacteria and the ways in which different groups obtain and use energy to survive. Students also need to understand biodiversity and its value.

  • This item is only available from Carolina Biological Supply Company.

Product Families Featuring This Item:

Components Qty Included?
Rid-X® Septic System Treatment (powder), 20 g 1 Included
Tetrazolium Indicator, 0.02%, 100 mL 1 Included
Corn Oil, 40 mL 1 Included
Graduated Plastic Pipets, 3 mL 81 Included
Cheesecloth, 20" 1 Included
Culture Tubes with Caps 64 Included
Plastic Medicine Cups, 1 oz 24 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Test Tube Rack (to hold culture tubes) 8 Needed, Not Included
Distilled Water, 200 mL 1 Needed, Not Included
Beaker, 250 mL 1 Needed, Not Included
Flask, 125 mL 1 Needed, Not Included
Funnel 1 Needed, Not Included
Glass Stirring Rod 1 Needed, Not Included
Pencils 16 Needed, Not Included
Nutrient Agar Plate (optional) 1 Optional, Not Included
Warm Tap Water, 140 mL 1 Needed, Not Included
Document Camera or Overhead Projector Needed, Not Included