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Addresses AP® Biology Big Ideas 1 and 3 and Essential Knowledge 1.A.1, 1.A.2, 3.A.1, 3.C.1, and 3.C.2. Students explore the process of transformation. They perform a classic transformation activity, to build background knowledge regarding plasmid function, genotype/phenotype connection, and antibiotic resistance. Using proper sterile technique, students then design an experiment to distinguish between 3 plasmids and determine their gene function. For a class of 32 students working in groups of 4.
Investigate the role of DNA as the determining source of heritable information and its impact on phenotypic traits.
Students discover and explore the process of transformation, a key technique in genetic engineering. They perform a classic transformation activity, to build background knowledge regarding plasmid function, genotype/phenotype connection, and antibiotic resistance. Using proper sterile technique and inquiry, students then design a new experiment to distinguish between 3 different plasmids and determine their gene function. The 8-Station Kit includes enough materials for a class of 32 students working in groups of 4. Kit includes prepaid coupon to request perishable materials later at your convenience.
AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, these products.
Components | Qty | Included? |
---|---|---|
Ampicillin, 10 mg/mL, 4 mL ![]() |
1 | Included |
Kanamycin, 4 mL ![]() |
1 | Included |
pGREEN Plasmid, 0.005 μg/μL, 200 μL ![]() |
1 | Included |
pAMP Plasmid, 0.005 μg/μL, 200 μL ![]() |
1 | Included |
pKAN Plasmid, 0.005 μg/μL, 200 μL ![]() |
1 | Included |
MM294 Slant Culture ![]() |
1 | Included |
Transformation Tubes | 16 | Included |
Glass Beads, 1 oz | 2 | Included |
Inoculating Loops | 28 | Included |
Sterile Pipettes, 1 mL | 56 | Included |
Nichrome Wire Inculating Loop | 1 | Included |
Petri Dishes | 60 | Included |
Luria Broth Agar, 400 mL | 3 | Included |
50 mM Calcium Chloride, 3 mL | 8 | Included |
Luria Broth, 3 mL | 8 | Included |
Teacher's Manual and Reproducible Student Guide | 1 | Included |
Microwave or Boiling Water Bath | 1 | Needed, Not included |
Bunsen Burner or Other Device for Flaming a Loop | 1 | Needed, Not included |
Water Bath | 1 | Needed, Not included |
Containers with Crushed Ice | Needed, Not included | |
Waste Containers | Needed, Not included | |
Thermometers | Needed, Not included | |
Household Bleach or Ethanol | Needed, Not included | |
Incubator (optional) | Needed, Not included |
With the current AP® Biology curriculum, you are no longer limited to 12 prescribed labs in your classroom—meaning you can now teach the course using labs that fit your classroom and student interests.
Carolina is proud to be the only supplier with unique kits and activities that are exclusively designed for the new AP® curriculum. Our Carolina Investigations® series is specifically aligned to the Big Ideas and Science Practices and was built from the ground up with the help of expert AP® teachers.
Focused on making inquiry easier in your classroom, Carolina Investigations® kits feature activities that allow students to take a guided approach to inquiry while building their Science Practices and connecting the Big Ideas. These kits offer an initial skill-building activity that features a more traditional classroom approach. This is followed by a secondary investigation using a unique experimental design template to help guide those student-driven, inquiry-based learning opportunities.
Catalog No. | Kit Title | Science Practices | Big Idea 1 | Big Idea 2 | Big Idea 3 | Big Idea 4 |
747500 | Origin of Life | 1, 2, 3, 4, 5, 6 | ✔ | |||
747510 | Population Genetics and Evolution | 2, 3, 4, 5, 7 | ✔ | |||
747520 | Natural Selection | 2, 3, 4, 5, 6, 7 | ✔ | |||
747600 | Cell Respiration | 1, 2, 3, 5, 6, 7 | ✔ | |||
747610 | Transpiration | 1, 2, 4, 6, 7 | ✔ | ✔ | ||
747620 | Physiology of the Circulatory System | 1, 2, 3, 6 | ✔ | |||
747700 | Animal Behavior | 2, 3, 4, 5, 6, 7 | ✔ | ✔ | ✔ | |
747710 | Electrophoresis and Simulated Genetic Screen | 1, 3, 5, 6, 7 | ✔ | |||
747720 | Exploring Mendelian Genetics | 1, 2, 3, 4, 6, 7 | ✔ | |||
747730 | Transformation | 1, 3, 4, 5, 6, 7 | ✔ | ✔ | ||
747740 | Cell Communication | 1, 3, 4, 5, 6, 7 | ✔ | ✔ | ||
747800 | Plant Pigments and Photosynthesis | 1, 2, 3, 6, 7 | ✔ | ✔ | ||
747810 | Species Interaction | 3, 5, 6, 7 | ✔ | |||
747820 | Evolving Enzymes | 1, 2, 3, 4, 5, 6, 7 | ✔ | ✔ | ✔ | ✔ |
181079 | Primary Consumer Energy Flow | 2, 3, 4, 5, 6, 7 | ✔ |
In addition to the Carolina Investigations® series, Carolina is proud to offer teachers the flexibility to use a variety of options in their classroom including:
In the end, whether you want to use one of our kits, follow a College Board® lab, or design your own experiment, Carolina has all the products and solutions you need for your AP® Biology classroom.
Let us help you help your students score a 5 on the AP® Exam!
AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, these products.
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