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Carolina Investigations® for Use with AP® Biology: Electrophoresis and Simulated Genetic Screen 1-Station Kit

$48.80
(in stock)

Description

Students conduct a simulated genetic screen using gel electrophoresis, which builds toward further understanding of heritability and the use of biotechnology. In this Guided Inquiry investigation, students assume the role of a lab technician tasked with performing a genetic screen to assess for the cystic fibrosis allele. Students learn how to pour and run an agarose gel, interpret a DNA fingerprint, and explore the techniques of PCR and restriction digests. Kit includes enough materials for 1 group.

Unit Connection
Unit 6: Gene Expression and Regulation, topic 6.8: Biotechnology

Standards Connection
IST-1 Heritable information provides for continuity of life.

Time Requirements
60 minutes of preparation before the Pre-Lab Guided Investigation. The Pre-Lab takes approximately 60 minutes, while the Guided Investigation takes approximately 3 60-minute class periods. The time required for the Inquiry Investigation will vary based on students' plans.

Digital Resources
Includes 1-year access to digital resources that support instruction. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, simulations, and post-lab analysis and assessments.

Prerequisite Knowledge and Skills
Students should be familiar with the concept of light as an energy source, the electromagnetic spectrum and the wavelengths of visible light, and the light-dependent reactions of photosynthesis.

AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, this product.

Specifications

What’s Included:
  • 1 Child #1 DNA, Tube
  • 1 Child #2 DNA, Tube
  • 1 Wild Type Control DNA, Tube
  • 1 Mutated Control DNA, Tube
  • 1 No DNA Control, Tube
  • HindIII/EcoRI Cut Lamda Marker
  • 1 Agarose, 5 g
  • 1 TAE Buffer, 50x, 100 mL
  • CarolinaBLU® Final Stain, 250 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 15 Pipet, Disposable Needle Point
  • 9 Staining Tray
  • 1 Teacher's Manual with Reproducible Student Guide
  • 1 Carolina Science Online® Resources (1-year subscription)
  • expand to see full list
Needed But Not Included:
  • 4 Gloves, Non-Latex
  • 1 Power Supply
  • 1 Electrophoresis Chamber with Tray
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