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Carolina Investigations® for Use with AP® Biology: Natural Selection 8-Station Kit



Students investigate natural genetic variation leading to variable survival rates in brine shrimp. Students place brine shrimp eggs in solutions of different salinity and measure hatching viability. Then students design and conduct an experiment to investigate further another factor that may impact hatching viability or survival rates of brine shrimp. Kit includes enough materials for 8 groups.

Unit Connection
Unit 7: Natural Selection, topics 7.2: Natural Selection and 7.3: Artificial Selection

Standards Connection
EVO-1 Evolution is characterized by a change in the genetic makeup of a population over time and is supported by multiple lines of evidence.

Time Requirements
The Pre-Lab takes approximately 60 minutes of preparation, the Guided Investigation takes approximately 55 minutes of preparation, and the Inquiry Investigation takes approximately 30 minutes of preparation.

The Pre-Lab takes approximately 45 minutes. The Guided Investigation takes approximately 30 minutes on Day 1, 15 minutes on Day 2, and 15 minutes on Day 3. The time required for the Inquiry Investigation will vary based on students' plans.

Digital Resources
Includes 1-year access to digital resources that support instruction. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, simulations, and post-lab analysis and assessments.

Prerequisite Knowledge and Skills
Students should be familiar with use of a stereomicroscope and preparation of solutions. They also should have a basic understanding Darwin’s theory of evolution by natural selection; a conceptual understanding of evolution as the change in the genetic makeup of a population over time; the biological species concept; and the influence of isolating mechanisms on the accumulation of genetic differences in a population, which may eventually lead to speciation.

AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, this product.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).


Shipping Information or Purchase Restrictions
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
What’s Included:
Needed But Not Included:
  • 1 Dechlorinated Water, 2.4 L
  • 40 Beakers, 40 mL
  • 8 Stirring Rods
  • 8 Small Plastic Bags
  • 8 Graduated Cylinders
  • 1 Scissors
  • 8 Laboratory Markers
  • 8 Stereomicroscopes
  • 1 Scale (measuring to 0.01 g)
  •  Weigh Boats
  •  Lab Spoons
  •  Thermometers (option for inquiry activity)
  •  Cooling Packs (option for inquiry activity)
  •  Heating Pads (option for inquiry activity)
  •  Sand (option for inquiry activity)
  •  Screens (option for inquiry activity)
  •  Lamp (option for inquiry activity)
  •  Aluminum Foil (option for inquiry activity)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.


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