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Carolina BioKits®: Exploring with Pectinase: An Introduction to Enzymes (with perishables)

(in stock)


For 32 students working cooperatively in 8 groups of 4. Introduce your students to enzymes and how temperature and concentration affect an enzyme's ability to convert reactants into products. In this activity, students add increasing amounts of the enzyme pectinase to applesauce and observe how much juice is released at various temperatures. They then mathematically depict the effect of increasing enzyme concentration by graphing the volume of enzyme used versus the volume of juice released. Note: This kit includes perishable materials for immediate use.


Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 1 Pectinase, 5 mL 
  • 30 Bulb Pipets, 3 mL
  • 40 Plastic Cups
  • 48 Wood Stirrers
  • 6 Applesauce, 1 lb
  • 45 Paper Cone Cups, 4 oz
  • 1 Cheesecloth, Pack
  • 1 Teacher’s Manual with Reproducible Student Guide
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Needed But Not Included:
  • 40 Graduated Cylinders (accurate to 1 mL), 50 or 100 mL
  • 6 Permanent Markers
  • 6 Containers for Distributing Pectinase (minimum 2 mL)
  • 6 Containers for Distributing Distilled or Deionized Water (minimum 2 mL)
  • 3 Tubs or Bins for Ice (large enough to accommodate 5 of the cups included in the kit)
  • 1 Masking or Lab Tape for Labeling Cups and Cylinders
  • 1 Container for Diluting Pectinase, 20 mL
  • 2 Scissors
  • 8 Ruler
  • 1 Distilled or Deionized Water, 50 mL
  • 3 Balances
  • 3 Spoons for Weighing Applesauce
  •  Graph Paper or Graphing Software
  •  Crushed Ice
  •  Timing Devices (1 per group)
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