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  • Restriction Mapping of Lambda DNA Kit with CarolinaBLU™ (with prepaid coupon)

Restriction Mapping of Lambda DNA Kit with CarolinaBLU™ (with prepaid coupon)

Item # 211173
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This kit provides data that allow students to assemble a map of cutting sites for ApaI, Eco0109I, and HindIII on the genome of the lambda virus. This is accomplished by digesting lambda DNA with the 3 restriction enzymes alone and in combination. A restriction map can be generated by using information gained from the electrophoresis of the lambda restriction fragments. Kit materials are sufficient for 6 teams of students or 6 separate instructor demonstrations.

Price

$175.00

In stock and available to ship.
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Overview

Advanced—For experienced high school and college classes; requires some technical skill.

This kit provides data that allow students to assemble a map of cutting sites for ApaI, Eco0109I, and HindIII on the genome of the lambda virus. This is accomplished by digesting lambda DNA with the 3 restriction enzymes alone and in combination. A restriction map can be generated by using information gained from the electrophoresis of the lambda restriction fragments. Kit materials are sufficient for 6 teams of students or 6 separate instructor demonstrations. Kit contains a prepaid coupon to request perishable materials later at your convenience. Contact us or return coupon to request delivery of perishables.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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What's Included

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Lambda DNA, 0.4 µg/µL, 50 µL 6 Included
Restriction Enzymes 3 Included
Restriction Buffer, 10x, 500 µL 1 Included
Loading Dye, 10x, 100 µL 6 Included
Electrophoresis Buffer, 150 mL 1 Included
CarolinaBLU™ Gel Stain, 7 mL 1 Included
CarolinaBLU™ Final Stain, 250 mL 1 Included
Agarose, 4 g 1 Included
Microcentrifuge Tubes 50 Included
Staining Trays 6 Included
Gloves 4 Included
Semi-Logarithmic Graph Paper 24 Included
Rulers 6 Included
Student Instructions 6 Included
Teacher’s Manual 1 Included
37° C and 35° C Water Baths Needed, Not Included
Gel Electrophoresis Chambers Needed, Not Included
Power Supplies Needed, Not Included
Gel Visualization Equipment (white light) Needed, Not Included
Micropipettor Needed, Not Included

Resources

Instruction Manuals & Buying Guides

How-To & Techniques

 

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DNA Learning Center

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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