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800.334.5551

DNA Restriction Analysis Kit with Ethidium Bromide (with perishables)

$128.65
(in stock)

Description

Advanced - For experienced high school and college classes; requires some technical skill.

This classic lab was developed in cooperation with the DNA Learning Center. Students perform restriction digests on bacteriophage lambda DNA, conduct electrophoretic analysis of the reactions, and stain the resulting gel. They then observe and analyze the stained patterns of fragments separated by size during gel electrophoresis.

Materials are sufficient for 6 teams of students or 6 separate instructor demonstrations. Kit contains perishable materials. Note: Store restriction enzymes in a freezer.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

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Shipping Information or Purchase Restrictions
  • Exclamation icon This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account. This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 6 Lambda DNA, 25 µL 
  • BamHI Restriction Enzyme, 25 µL 
  • HindIII Restriction Enzyme, 25 µL 
  • EcoRI Restriction Enzyme, 25 µL 
  • 1 Restriction Buffer 
  • 1 TBE Buffer, 150 mL
  • 6 Loading Dye, 100 µL
  • 1 Ethidium Bromide Stain, 250 mL
  • 1 Agarose, 3.2 g
  • 1 Potassium Permanganate, 2%, 250 mL
  • 40 Microcentrifuge Tubes
  • 6 Staining Trays
  • 4 Disposable Gloves
  • 2 Student Instructions
  • 1 Teacher's Manual
  • expand to see full list
Needed But Not Included:
  •  Water Bath (37° C)
  •  Micropipettor
  •  Gel Electrophoresis Chamber
  •  Power Supply
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