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DNALC Forensic DNA Fingerprinting Kit with CarolinaBLU® (with voucher)

$164.70

Description

Advanced—For experienced high school and college classes; requires some technical skill.

Developed in cooperation with the DNA Learning Center, this advanced lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. The resulting "DNA fingerprints" of the suspects are compared for a match with the evidence. Materials are sufficient for 8 groups of 4 students. Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishables. Foreign orders require special handling.

FREE 1-year access to digital resources that support NGSS instruction included.

FREE 1-year access to digital resources that support NGSS instruction included.

Specifications

Shipping Information or Purchase Restrictions
  • Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.
What’s Included:
  • E. coli Slant Cultures (voucher item)
  • 1 Restriction Buffer, 180 µL (voucher item)
  • 1 Restriction Enzyme Mixture, 85 µL (voucher item)
  • 1 RNase A, 250 µL, 5 µL/mL (voucher item)
  • 1 Evidence A DNA, 80 µL, 0.1 µg/µL (voucher item)
  • 1 Evidence B DNA, 80 µL, 0.1 µg/µL (voucher item)
  • 4 Luria Broth, 10 mL
  • 2 Glucose-Tris-EDTA, 3.5 mL
  • 2 Potassium Acetate-Acetic Acid Solution, 3.5 mL
  • 2 Isopropanol, 100%, 10 mL
  • 1 Ethanol, 95%, 15 mL
  • 1 Tris-EDTA Buffer, 3.5 mL
  • 1 Sodium Dodecyl Sulfate Solution, 10%, 1 mL
  • 1 0.22 N Sodium Hydroxide Solution, 8 mL
  • 1 Loading Dye, 1 mL
  • 1 TBE Electrophoresis Buffer, 20x, 200 mL
  • 1 Agarose, 5 g
  • 1 Sterile Distilled Water, 1 mL
  • CarolinaBLU® Final Stain, 400 mL
  • CarolinaBLU® Gel and Buffer Stain, 14 mL
  • 130 Microcentrifuge Tubes, 1.5 mL
  • 8 Staining Trays
  • 100 Transfer Pipets
  • 16 Disposable Gloves
  • 4 Inoculating Loops
  • 1 Teacher’s Manual with Reproducible Student Guide
Needed But Not Included:
  •  Document Camera or Overhead Projector
  •  Water Bath, 37° C
  •  Water Bath, 60° C (optional)
  •  Microcentrifuge (that generates at least 2,300 x g)
  •  Gel Electrophoresis Chambers (with casting trays and well-forming combs)
  •  Electrophoresis Power Supplies
  •  White Light Transilluminator (for viewing gels)
  •  Micropipets (for 1- to 1,000-µL volumes)
  •  Masking Tape (for sealing gel casting tray)
  • 8 Racks (for 1.5-mL centrifuge tubes)
  •  Flask or Beaker, 1 L (for agarose)
  • 8 Beakers or Similar Containers, 600 mL (for ice)
  • 8 Beakers or Similar Containers, 600 mL (for waste, such as used pipets)
  •  Carboy or Container, 4 L or Larger (for electrophoresis buffer)
  •  Ice (preferably crushed)
  •  Distilled Water
  •  Permanent Markers
  •  Boiling Water Bath or Microwave Oven
  •  Autoclave or 10% Household Bleach Solution (for decontamination of bacterial waste before disposal)
  •  Disinfectant (10% household bleach solution, 70% ethanol, isopropanol, or other for cleaning lab surfaces)
  •  Paper Towels
  •  Camera (for recording results, optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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