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Detecting Epigenetic DNA Methylation in Arabidopsis Amplification and Electrophoresis Kit with CarolinaBLU® Stain (with voucher)

$312.35
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Introduce students to the growing field of epigenetics
  • Gain experience working with the model plant Arabidopsis thaliana
  • Investigate the role of DNA methylation in gene regulation
  • Use a protocol designed especially for the classroom
Utilize methylation-sensitive enzymes to explore epigenetics—heritable changes in gene expression—that affect flowering in Arabidopsis. By growing wild-type Ler and mutant fwa-1 plants, students are able to observe phenotypic differences in flowering and then investigate this difference using multiple molecular techniques. Students conduct DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics to investigate the pivotal role DNA methylation plays in gene regulation. Kit contains materials for 25 DNA extractions and PCR materials for electrophoresis using CarolinaBLU®. Note: Kit includes a voucher to request perishables later at your convenience. Contact us or return the voucher to request delivery of perishable materials. Kit must be used within 4 weeks of receiving it.

Specifications

What’s Included:
  • Arabidopsis Seed Pack from Ler (Landsberg erecta) Wild Type (voucher item)
  • Arabidopsis Seed Pack from fwa-1 (flowering wageningen) Mutant (voucher item)
  • FWA Primer/Loading Dye Mix, 700 µL (voucher item)
  • 1 McrBc Buffer, 10x, 200 µL (voucher item)
  • 1 McrBc Enzyme, 10 U/µL, 20 µL (voucher item)
  • 1 Guanosine 5′–Triphosphate (GTP), 100 mM, 15 µL (voucher item)
  • 1 BSA, 10 mg/mL, 30 µL (voucher item)
  • 1 Sterile Distilled Water, 1 mL (voucher item)
  • 1 RNAse Solution, 5 mg/mL, 250 µL (voucher item)
  • 1 Potting Soil, Bag (voucher item)
  • 1 pBR322/BstNI Markers, 0.075 µg/µL, 130 µL (voucher item)
  • 25 Ready-to-Go™ PCR Beads, 0.2-mL Tubes (voucher item)
  • 1 Nuclei Lysis Solution, 12 mL
  • 1 Protein Precipitation Solution, 3.5 mL
  • 2 Isopropanol, 100%, 20 mL
  • 1 Ethanol, 70%, 30 mL
  • 1 DNA Rehydration Buffer, 2.5 mL
  • 15 Plastic Pestles
  • 1 Mineral Oil, 5 mL
  • 2 Agarose, 4 g
  • 1 20x TBE Buffer, 150 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • CarolinaBLU® Final Stain, 250 mL
  • 12 Latex Gloves
  • 6 Staining Trays
  • 1 Planting Flat and Tray
  • 1 Plastic Dome Lid
  • 1 Teacher’s Manual with Reproducible Student Guide
Needed But Not Included:
  •  Tweezers, Scissors, or Scalpel
  •  Water Baths/Heating Blocks (65° C and 37° C)
  •  Micropipets and Tips, 1 to 1,000 µL
  •  Microcentrifuge
  • 32 Microcentrifuge Tubes, 1.5 mL
  • 8 Microcentrifuge Tube Racks
  • 1 Thermal Cycler, Programmable
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Container with Ice
  •  Computer with Internet Access
  •  Microwave, Hot Plate, or Boiling Water
  •  Vortexer (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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