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  • Detecting Epigenetic DNA Methylation in Arabidopsis Amplification and Electrophoresis Kit with GelGreen™ Stain (with perishables)

Detecting Epigenetic DNA Methylation in Arabidopsis Amplification and Electrophoresis Kit with GelGreen™ Stain (with perishables)

Item # 211405P Exclusive
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Use methylation-sensitive enzymes to explore epigenetics—heritable changes in gene expression—that affect flowering in Arabidopsis. Using techniques such as DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics, students learn the pivotal role DNA methylation plays in gene expression. Each kit contains materials for 25 DNA extractions and PCR reactions and materials for electrophoresis using GelGreen™.

Price

$362.00

In stock and available to ship.
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Overview

Advanced—For experienced high school and college classes; requires some technical skill.

  • Introduce students to the growing field of epigenetics
  • Gain experience working with the model plant Arabidopsis thaliana
  • Investigate the role of DNA methylation in gene regulation
  • Use a protocol designed especially for the classroom
Utilize methylation-sensitive enzymes to explore epigenetics—heritable changes in gene expression—that affect flowering in Arabidopsis. By growing wild-type Ler and mutant fwa-1 plants, students are able to observe phenotypic differences in flowering and then investigate this difference using multiple molecular techniques. Students conduct DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics to investigate the pivotal role DNA methylation plays in gene regulation. Kit contains materials for 25 DNA extractions and PCR materials for electrophoresis using GelGreen™. Note: Kit includes perishable materials. Kit must be used within 4 weeks of receiving it.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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What's Included

This item ships with living or perishable materials (all   items). Please select a delivery date during checkout.

Components Qty Included?
Arabidopsis Seed Pack from Ler (Landsberg erecta) Wild Type  1 Included
Arabidopsis Seed Pack from fwa-1 (flowering wageningen) Mutant  1 Included
FWA Primer/Loading Dye Mix, 700 µL  1 Included
McrBc Buffer, 10x, 200 µL  1 Included
McrBc Enzyme, 10 U/µL, 20 µL  1 Included
Guanosine 5′–Triphosphate (GTP), 100 mM, 15 µL  1 Included
BSA, 10 mg/mL, 30 µL  1 Included
Sterile Distilled Water, 1 mL  1 Included
RNAse Solution, 5 mg/mL, 250 µL  1 Included
Potting Soil, Bag  1 Included
pBR322/BstNI Markers, 0.075 µg/µL, 130 µL  1 Included
Ready-to-Go™ PCR Beads, 0.2-mL Tubes  25 Included
Nuclei Lysis Solution, 12 mL 1 Included
Protein Precipitation Solution, 3.5 mL 1 Included
Isopropanol, 100%, 20 mL 2 Included
Ethanol, 70%, 30 mL 1 Included
DNA Rehydration Buffer, 2.5 mL 1 Included
Plastic Pestles 15 Included
Mineral Oil, 5 mL 1 Included
Agarose, 4 g 2 Included
20x TBE Buffer, 150 mL 1 Included
GelGreen™ Nucleic Acid Gel Stain, 100 µL 1 Included
NaCl, 5 M, 8 mL 1 Included
Latex Gloves 12 Included
Staining Trays 6 Included
Planting Flat and Tray 1 Included
Plastic Dome Lid 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Tweezers, Scissors, or Scalpel Needed, Not Included
Water Baths/Heating Blocks (65° C and 37° C) Needed, Not Included
Micropipets and Tips, 1 to 1,000 µL Needed, Not Included
Microcentrifuge Needed, Not Included
Microcentrifuge Tubes, 1.5 mL 32 Needed, Not Included
Microcentrifuge Tube Racks 8 Needed, Not Included
Thermal Cycler, Programmable 1 Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Container with Ice Needed, Not Included
Computer with Internet Access Needed, Not Included
Microwave, Hot Plate, or Boiling Water Needed, Not Included
Vortexer (optional) Needed, Not Included

Resources

How-To & Techniques

Sample Teacher Manuals

 

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DNA Learning Center

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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