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Mitochondrial DNA Polymorphisms in Human Evolution DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® Stain and 0.2-mL Tubes (with voucher)

$212.50
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

Generate excitement by having your students amplify their own mitochondrial DNA for sequencing!

  • Uses a simple, robust protocol ideal for the classroom
  • Enlivens discussions on the molecular basis of heredity and evolution
  • Provides a basic version of the lab that is used in forensics and the analysis of ancient DNA samples
  • Teaches real-world applications of PCR
  • Includes online resources to enhance the learning experience

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome, which contains numerous single nucleotide polymorphisms (SNPs). After gel electrophoresis confirms amplification, the student samples may be sent for sequencing (the cost of sequencing is not included in the price of the kit). Students then compare their SNPs to ancient hominids and to people from different world populations to discover patterns of DNA variation and to analyze theories of human evolution.

Kit contains instructions and materials for 25 students to perform DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU®). Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.

Specifications

What’s Included:
  • 1 mtDNA Primer/Loading Dye Mix, 700 µL (voucher item)
  • 1 pBr322/BstNI Markers, 0.075 µg/µL, 130 µL (voucher item)
  • 1 Proteinase K, 100 µg/mL, 5 mL (voucher item)
  • 1 Chelex® 100 Resin, 0.5 g
  • 25 PCR Tubes Containing Ready-to-Go™ Beads (0.2-mL capacity)
  • 1 Mineral Oil, 5 mL
  • 1 Agarose, 5 g
  • 1 TBE Buffer, 150 mL
  • CarolinaBLU® Final Stain, 250 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 4 Latex Gloves
  • 6 Staining Trays
  • 1 Teacher's Manual with Reproducible Student Guide
Needed But Not Included:
  •  Aluminum Foil
  •  Beakers Containing Ice
  •  Programmable DNA Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Forceps
  •  Laboratory Markers (1 per student)
  •  Micropipets and Tips, 100–1,000 µL
  •  Micropipets and Tips, 1–10 µL or 1–20 µL
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge Tubes, 1.5 mL
  •  Paper Cups
  •  Razor Blade or Scalpel
  •  Saline Solution, 0.9%
  •  Boiling Water Baths
  •  Water Baths, 37° and 65° C
  •  White Light Box (for CarolinaBLU® stain)
  •  Vortexer (optional)
  •  Centrifuge for 15-mL Tubes (minimum 500 x g; optional but useful)
  •  Centrifuge for 1.5-mL Tubes (optional but useful)
  •  Camera (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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