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800.334.5551

Human Alu DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® Stain and 0.2-mL Tubes (with perishables)

$221.10
Product Highlights
  • Students extract their own DNA and determine their own genotype
  • Make real-world connections between population genetics, evolution, and more
  • Additional Alu PV92 primers available separately
(in stock)

Description

Product Details
Advanced—For experienced high school and college classes; requires some technical skill.

This experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies. Students will:

  • Use safe saline mouthwash and Chelex® extraction to obtain their own DNA sample
  • Amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene
  • Use electrophoresis to separate the 2 alleles
  • Each student determines his/her own genotype

Note: Kit is shipped with perishable materials.

Product Features

  • Explains population genetics and evolution using PCR technology
  • Incorporates Hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Students discover how Alu insertions are used to track world population migrations
  • With PuReTaq™ Ready-to-Go™ PCR Beads
  • Includes electrophoresis reagents
  • With CarolinaBLU® stain
  • Kit equips 25 students

Digital Resources
Includes 1-year access to digital resources that include a teacher’s manual, student guide, and more.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 1 PV92B Primer/Loading Dye Mix, 700 µL 
  • 1 pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL 
  • 1 Proteinase K, 100 µg/mL, 5 mL 
  • 1 Chelex® Resin, 0.5 g
  • 25 PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity)
  • 1 Mineral Oil, 5 mL
  • 1 Agarose, 5 g
  • 1 TBE, 150 mL
  • CarolinaBLU® Final Stain, 250 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 4 Latex Gloves
  • 6 Staining Trays
  • Alu CD-ROM
  • 1 Teacher's Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Saline Solution, 0.9% (10 mL per student)
  •  Micropipets and Tips (1 µL to 1,000 µL)
  •  Microcentrifuge Tubes, 1.5 mL (2 per student)
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL (1 per student)
  •  Tube Adapters (for microcentrifuge)
  •  Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Gel Staining Trays
  •  White Light Box (for CarolinaBLU® stain)
  •  Paper Cup (1 per student)
  •  Permanent Markers
  •  Container of Crushed Ice
  •  Camera (optional)
  •  Boiling Water Bath (optional)
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Return Policy:

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