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Human Alu DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® Stain and 0.2-mL Tubes (with voucher)

$221.10
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Simple, robust protocol is ideal for classroom use
  • Incorporates Hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Discover how Alu insertions are used to track world population migrations
  • CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles, and each student determines his/her own genotype. The experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies.

Kit contains instructions, a CD-ROM, and materials for 25 students to perform DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU®). Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.

Specifications

What’s Included:
  • PV92B Primer/Loading Dye Mix, 700 µL (voucher item)
  • 1 pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL (voucher item)
  • 1 Proteinase K, 100 µg/mL, 5 mL (voucher item)
  • 1 Chelex® Resin, 0.5 g
  • 25 PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity)
  • 1 Mineral Oil, 5 mL
  • 1 Agarose, 5 g
  • 1 TBE, 150 mL
  • CarolinaBLU® Final Stain, 250 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 4 Latex Gloves
  • 6 Staining Trays
  • Alu CD-ROM
  • 1 Teacher's Manual with Reproducible Student Guide
Needed But Not Included:
  •  Saline Solution, 0.9% (10 mL per student)
  •  Micropipets and Tips (1 µL to 1,000 µL)
  •  Microcentrifuge Tubes, 1.5 mL (2 per student)
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL (1 per student)
  •  Tube Adapters (for microcentrifuge)
  •  Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Gel Staining Trays
  •  White Light Box (for CarolinaBLU® stain)
  •  Paper Cup (1 per student)
  •  Permanent Markers
  •  Container of Crushed Ice
  •  Camera (optional)
  •  Boiling Water Bath (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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